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Lack of association between interleukin-10, transforming growth factor-beta gene polymorphisms and juvenile-onset systemic lupus erythematosus

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Abstract

As abundant types of genetic predisposition and environmental factors seem to be associated with the development of juvenile-onset systemic lupus erythematosus (JSLE), we investigated the gene polymorphisms of two anti-inflammatory cytokines, including interleukin-10 (IL-10) and transforming growth factor-beta (TGF-β), which were previously found to be associated with SLE in adults, in a group of patients with JSLE. We studied a group of 59 Iranian patients with JSLE in comparison with 140 healthy controls and assessed the frequency of alleles, genotypes, and haplotypes of IL-10 and TGF-β single-nucleotide polymorphisms (SNPs) using polymerase chain reaction with sequence-specific primers method. The CA genotype was significantly more frequent at position −592 in IL-10 in patients with juvenile-onset systemic lupus erythematosus than in the controls (P = 0.01). Genotype CC was detected at the same position in 32.7 % of the patients; this frequency was significantly lower than the frequency of 50.7 % recorded in the healthy controls (P = 0.03). The TC haplotype of TGF-β (codon 10, codon 25) was significantly more frequent in the patients with juvenile-onset systemic lupus erythematosus than in the healthy controls (P = 0.02). Nevertheless, these significant associations disappear after Bonferroni correction. Our findings suggest that IL-10 (−1082, −819, −592) and TGF-β (codon 10, codon 25) gene variants may not be associated with the development of JSLE in Iranian population.

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Acknowledgments

This study was supported by a grant (no. 18261) from Tehran University of Medical Sciences.

Conflicts of interest

The authors report no conflicts of interest.

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Authors and Affiliations

  1. Research Center for Immunodeficiencies, Children’s Medical Center, Tehran University of Medical Sciences, Dr Qarib St, Keshavarz Blvd, Tehran, 14194, Iran

    Arezou Rezaei, Sara Harsini & Nima Rezaei

  2. Pediatric Rheumatology Research Group, Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Vahid Ziaee

  3. Pediatrics Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

    Vahid Ziaee, Fatemeh Tahghighi Sharabian, Mohammad Hassan Moradinejad & Nima Rezaei

  4. School of Nutrition and Dietetics, Tehran University of Medical Sciences, Tehran, Iran

    Maryam Mahmoudi

  5. Molecular Immunology Research Center and Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Samaneh Soltani, Maryam Sadr & Nima Rezaei

  6. Department of Pediatrics, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Yahya Aghighi

  7. Universal Scientific Education and Research Network (USERN), Tehran, Iran

    Nima Rezaei

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  1. Arezou Rezaei
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  2. Vahid Ziaee
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Correspondence to Nima Rezaei.

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Rezaei, A., Ziaee, V., Sharabian, F.T. et al. Lack of association between interleukin-10, transforming growth factor-beta gene polymorphisms and juvenile-onset systemic lupus erythematosus. Clin Rheumatol 34, 1059–1064 (2015). https://doi.org/10.1007/s10067-015-2877-2

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