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Interleukin-6, interleukin-1 gene cluster and interleukin-1 receptor polymorphisms in Iranian patients with juvenile systemic lupus erythematosus

  • Research Article
  • Published:
European Cytokine Network

Abstract

Background

Juvenile systemic lupus erythematosus (JSLE) is a polygenic, autoimmune disorder of unknown origin. As proinflammatory cytokines, including interleukin-6 (IL-6) and the interleukin-1 (IL-1) family, seem to contribute to the pathogenesis of JSLE, this investigation was performed to assess the associations of particular single nucleotide polymorphisms (SNPs) of IL-6 and IL-1 genes in a case-control study.

Methods

Fifty nine JSLE cases were recruited for this study as the patient group, and were compared against 140 healthy, unrelated, control subjects. Using the polymerase chain reaction with the sequence-specific primer method, genotyping was carried out for the IL-6 gene at positions −174 and nt565, as well as the IL-1α gene at position −889, the IL-1β gene at positions −511 and +3962, the interleukin-1 receptor (IL-1R) gene at position Pst-I 1970, and the interleukin-1 receptor antagonist (IL-1Ra) gene at position Mspa-I 11100.

Results

Results of the analyzed data revealed a remarkable, positive association for the promoter sequence of the IL-1β gene at position −511 for T/T in the patient group compared with healthy controls (P value, 0.03). Furthermore, a significant negative association was found between the T/C genotype at the same position on the IL-1β gene in juvenile SLE (P value, 0.03).

Conclusions

cytokine gene polymorphisms might play a role in the pathophysiology of JSLE. Particular IL-1 gene variants could affect individual susceptibility to JSLE.

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Author information

Authors and Affiliations

  1. Pediatric Rheumatology Research Group, Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Vahid Ziaee & Fatemeh Tahghighi

  2. Pediatric Center of Excellence, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

    Vahid Ziaee, Fatemeh Tahghighi, Mohammad Hassan Moradinejad & Nima Rezaei

  3. Research Center for Immunodeficiencies, Children’s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

    Sara Harsini, Arezou Rezaei & Nima Rezaei

  4. School of Nutrition and Dietetics, Tehran University of Medical Sciences, Tehran, Iran

    Maryam Mahmoudi

  5. Molecular Immunology Research Center and Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Samaneh Soltani, Maryam Sadr & Nima Rezaei

  6. Department of Pediatrics, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Yahya Aghighi

  7. Research Center for Immunodeficiencies, Children’s Medical Center Hospital, Dr Qarib St, Keshavarz Blvd, Tehran, 14194, Iran

    Nima Rezaei

Authors
  1. Vahid Ziaee
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  2. Fatemeh Tahghighi
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Correspondence to Nima Rezaei.

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Ziaee, V., Tahghighi, F., Moradinejad, M.H. et al. Interleukin-6, interleukin-1 gene cluster and interleukin-1 receptor polymorphisms in Iranian patients with juvenile systemic lupus erythematosus. Eur Cytokine Netw 25, 35–40 (2014). https://doi.org/10.1684/ecn.2014.0352

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  • DOI: https://doi.org/10.1684/ecn.2014.0352

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