Abstract
We present the result of our research on the tumorigenic ability of single cell clones isolated from an aggressive murine breast cancer cell line in a matched allografting mouse model. Tumor formation is basically dependent on the cell numbers injected per location. We argue that in vivo tumor formation from single cell clones, isolated in vivo from cancer cell lines, may not provide conclusive evidence to disprove the cancer stem cell (CSC) theory without additional data.
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References
Danovi, S.A. (2009). Cancer stem cells: common as muck. Nat. Rev. Cancer 9, 6–7.
Ewens, A., Luo, L., Berleth, E., Alderfer, J., Wollman, R., Hafeez, B.B., Kanter, P., Mihich, E., and Ehrke, M.J. (2006). Doxorubicin plus interleukin-2 chemoimmunotherapy against breast cancer in mice. Cancer Res. 66, 5419–5426.
Quintana, E., Shackleton, M., Sabel, M.S., Fullen, D.R., Johnson, T.M., and Morrison, S.J. (2008). Efficient tumour formation by single human melanoma cells. Nature 456, 593–598.
Visvader, J.E., and Lindeman, G.J. (2008). Cancer stem cells in solid tumours: accumulating evidence and unresolved questions. Nat. Rev. Cancer 8, 755–768.
Yoo, M.H., and Hatfield, D.L. (2008). The cancer stem cell theory: Is it correct? Mol. Cells 26, 514–516.
Zheng, X., Shen, G., Yang, X., and Liu, W. (2007). Most C6 cells are cancer stem cells: evidence from clonal and population analyses. Cancer Res. 67, 3691–3697.
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Li, F. Every single cell clones from cancer cell lines growing tumors in vivo may not invalidate the cancer stem cell concept. Mol Cells 27, 491–492 (2009). https://doi.org/10.1007/s10059-009-0056-5
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DOI: https://doi.org/10.1007/s10059-009-0056-5