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Limiting Dilution Tumor Initiation Assay: An In Vivo Approach for the Study of Cancer Stem Cells

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Stem Cell Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2429))

Abstract

Cancer stem cells (CSCs) are a small subpopulation of self-renewing cancer cells that are present within tumors. Calculating the frequency of tumor-initiating cells is important in the assessment of the number of CSCs present in a cell population. In this chapter, we present a protocol developed for quantification of CSCs from breast cancer tumors that can be adapted to CSCs from other types of tumors.

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References

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Acknowledgments

This work was supported by the NIH/NCI grants (2R01CA155243; 1R01CA200970), the CPRIT grant (RP170172) awarded to S.A.M, and the NIH T32 grant (5T32CA18682) awarded to N.A.K.

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Authors and Affiliations

  1. Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Petra den Hollander, Robiya Joseph, Suhas Vasaikar, Nick A. Kuburich, Abhijeet P. Deshmukh & Sendurai A. Mani

Authors
  1. Petra den Hollander
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  2. Robiya Joseph
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  3. Suhas Vasaikar
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  4. Nick A. Kuburich
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  5. Abhijeet P. Deshmukh
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  6. Sendurai A. Mani
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Corresponding author

Correspondence to Sendurai A. Mani .

Editor information

Editors and Affiliations

  1. Stem Cell and Cancer Biology Laboratory, Division of Experimental Medicine and Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA

    Nagarajan Kannan

  2. Hull York Medical School, York, UK

    Philip Beer

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den Hollander, P., Joseph, R., Vasaikar, S., Kuburich, N.A., Deshmukh, A.P., Mani, S.A. (2022). Limiting Dilution Tumor Initiation Assay: An In Vivo Approach for the Study of Cancer Stem Cells. In: Kannan, N., Beer, P. (eds) Stem Cell Assays. Methods in Molecular Biology, vol 2429. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1979-7_38

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  • DOI: https://doi.org/10.1007/978-1-0716-1979-7_38

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1978-0

  • Online ISBN: 978-1-0716-1979-7

  • eBook Packages: Springer Protocols

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