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Characterization and electrochemical study of hemoglobin–carbon nanoparticles–polyvinyl alcohol nanoporous hybrid film

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Abstract

A hybrid film is fabricated by casting hemoglobin (Hb)–carbon nanoparticles (CNPs)–polyvinyl alcohol (PVA) suspension on glassy carbon electrode (GCE). The resulting film shows a three-dimensional nanoporous structure. In the hybrid film, the ultraviolet visible (UV–Vis) absorption spectra of Hb keep almost unchanged. The organic–inorganic hybrid material can promote the direct electron transfer of Hb. A pair of well-defined and quasireversible peaks with a formal potential of −0.348 V (vs saturated calomel electrode) is obtained, which is caused by the electrochemical reaction of the Fe(III)/Fe(II) couple of Hb. The electron transfer rate constant (k s) is estimated to be 3.9 s−1. The immobilized Hb exhibits high stability and excellent electrochemical catalysis to the reduction of oxygen (O2), hydrogen peroxide (H2O2), and nitrite (\(NO^{ - }_{2} \)). The catalytic currents are linear to the concentrations of H2O2 and \(NO^{ - }_{2} \) from 1.96 to 112 μM and from 0.2 to 1.8 mM, respectively. Therefore, the hybrid film may be a good matrix for protein immobilization and biosensor fabrication.

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Acknowledgements

The authors appreciate the support from the National Nature Science Foundation of China (Grant No. 20173040).

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Authors and Affiliations

  1. Department of Chemistry, Wuhan University, Wuhan, 430072, People’s Republic of China

    Jing-Jing Yu, Fa-Qiong Zhao & Bai-Zhao Zeng

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  1. Jing-Jing Yu
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  2. Fa-Qiong Zhao
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  3. Bai-Zhao Zeng
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Correspondence to Bai-Zhao Zeng.

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Yu, JJ., Zhao, FQ. & Zeng, BZ. Characterization and electrochemical study of hemoglobin–carbon nanoparticles–polyvinyl alcohol nanoporous hybrid film. J Solid State Electrochem 12, 1167–1172 (2008). https://doi.org/10.1007/s10008-007-0467-1

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  • DOI: https://doi.org/10.1007/s10008-007-0467-1

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