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Description of gamma radiation-resistant Geodermatophilus dictyosporus sp. nov. to accommodate the not validly named Geodermatophilus obscurus subsp. dictyosporus (Luedemann, 1968)

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Abstract

A gamma radiation-resistant, Gram reaction-positive, aerobic and chemoorganotrophic actinobacterium, initially designated Geodermatophilus obscurus subsp. dictyosporus G-5T, was not validly named at the time of initial publication (1968). G-5T formed black-colored colonies on GYM agar. The optimal growth range was 25–35 °C, at pH 6.5–9.5 and in the absence of NaCl. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G + C content of the strain was 75.3 mol  %. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diamino acid. The main polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and one unspecified glycolipid; MK-9(H4) was the dominant menaquinone and galactose was detected as a diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids, iso-C16:0 and iso-C15:0. The 16S rRNA gene showed 94.8–98.4 % sequence identity with the members of the genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G-5T is proposed to represent a novel species, Geodermatophilus dictyosporus and the type strain is G-5T (=DSM 43161T = CCUG 62970T = MTCC 11558T = ATCC 25080T = CBS 234.69T = IFO 13317T = KCC A-0154T = NBRC 13317T). The INSDC accession number is HF970584.

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Acknowledgments

We would like to gratefully acknowledge the help of Gabrielle Pötter for assistance with chemotaxonomy, Brian J. Tindall (all at DSMZ, Braunschweig) for his guidance in the chemotaxonomic analyses and Maher Gtari (ISSTE, Tunisia) for his collaboration in the gamma irradiation experiments. M.C. Montero-Calasanz is the recipient of a DSMZ postdoctoral fellowship 2013–2015.

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Authors and Affiliations

  1. Leibniz Institute DSMZ, German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany

    Maria del Carmen Montero-Calasanz, Markus Göker, Cathrin Spröer, Peter Schumann & Hans-Peter Klenk

  2. Laboratoire Microorganismes et Biomolécules Actives, Université de Tunis Elmanar (FST) et Université de Carthage (ISSTE), 2092, Tunis, Tunisia

    Karima Hezbri

  3. National Center for Nuclear Sciences and Technology (CNSTN), Sidi Thabet Technopark, 2020, Ariana, Tunisia

    Haïtham Sghaier

  4. HZI, Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124, Braunschweig, Germany

    Manfred Rohde

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  1. Maria del Carmen Montero-Calasanz
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Correspondence to Maria del Carmen Montero-Calasanz or Hans-Peter Klenk.

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Communicated by M. da Costa.

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792_2014_708_MOESM1_ESM.tif

Supplementary material 1 (TIFF 1661 kb). Supplementary Fig. S1. Scanning electron micrograph of strain G-5T grown on GYM medium for 7 days at 28 °C

792_2014_708_MOESM2_ESM.pdf

Supplementary material 2 (PDF 45 kb). Supplementary Fig. S2. The parameter “Maximum Height” estimated from the respiration curves as measured with the OmniLog phenotyping device and discretized and visualized as heatmap using the opm package. Plates and substrates are rearranged according to their overall similarity (as depicted using the row and column dendrograms). Ocher color indicates positive reaction; purple color indicates negative reaction; green color indicates ambiguous reaction. Letters (A/B) indicate each replicate of experiment

792_2014_708_MOESM3_ESM.tif

Supplementary material 3 (TIFF 326 kb). Supplementary Fig. S3. Polar lipids profile of Geodermatophilus dictyosporus sp. nov. G-5T, after separation by two-dimensional TLC. Plate was sprayed with molydatophosphoric acid for detection of total polar lipid. DPG, diphosphadidylglycerol; PE, phosphatidethanolamine; PC, phosphatidylcholine; PI, phosphatidylinositol; GL, unknown glycolipid

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Montero-Calasanz, M.d.C., Hezbri, K., Göker, M. et al. Description of gamma radiation-resistant Geodermatophilus dictyosporus sp. nov. to accommodate the not validly named Geodermatophilus obscurus subsp. dictyosporus (Luedemann, 1968). Extremophiles 19, 77–85 (2015). https://doi.org/10.1007/s00792-014-0708-z

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