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Effects of ionizing radiation on woven bone: influence on the osteocyte lacunar network, collagen maturation, and microarchitecture

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Abstract

Objectives

Evaluate the effects of ionizing radiation on microarchitecture, the osteocyte lacunar network, and collagen maturity in a bone repair site.

Materials and methods

Bone defects were created on tibias of 20 New Zealand rabbits. After 2 weeks, the animals were randomly divided into (n = 10) NoIr (nonirradiated group) and Ir (irradiated group). In the Ir, the animals received single-dose irradiation of 30 Gy on the tibia and were euthanized after 2 weeks. Bone microarchitecture parameters were analyzed by using micro-CT, and the osteocyte lacunar network, bone matrix, and collagen maturation by histomorphometric analysis. The data were analyzed using unpaired Student’s t test (α = 0.05).

Results

Trabecular thickness in Ir was lower than that in NoIr (P = 0.028). No difference was found for bone volume fraction and bone area. Lacunae filled with osteocytes were more numerous (P < 0.0001) in NoIr (2.6 ± 0.6) than in Ir (1.97 ± 0.53). Empty lacunae were more prevalent (P < 0.003) in Ir (0.14 ± 0.10) than in NoIr (0.1 ± 0.1). The mean osteocyte lacunae size was higher (P < 0.01) in Ir (15.4 ± 4.41) than in NoIr (12.7 ± 3.7). Picrosirius red analysis showed more (P < 0.05) mature collagen in NoIr (29.0 ± 5.3) than in Ir (23.4 ± 4.5). Immature collagen quantification revealed no difference between groups.

Conclusions

Ionizing radiation compromised bone formation and an impairment in bone repair in irradiated woven bone was observed.

Clinical relevance

Before radiotherapy, patients usually need surgical intervention, which may be better performed, if clinicians understand the repair process in irradiated bone, using novel approaches for treating these individuals.

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Acknowledgments

The authors are grateful to the Department of Clinical Oncology at Federal University of Triângulo Mineiro (UFTM), MG, Brazil.

Funding

This study was financed in part by Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001.

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Authors and Affiliations

  1. Department of Periodontology and Implantology, School of Dentistry, Federal University of Uberlândia, Avenida Pará s/n°, Campus Umuarama, Bloco 4L, Bairro Umuarama, Uberlândia, MG, 38400-902, Brazil

    Eduardo Moura Mendes, Milena Suemi Irie, Juliana Simeão Borges & Priscilla Barbosa Ferreira Soares

  2. Dentistry Department, Federal University of Santa Catarina, Rua Delfino Conti SN, Trindade, Florianópolis, SC, 88040-370, Brazil

    Gustavo Davi Rabelo

  3. Institute of Biomedical Sciences, Federal University of Uberlândia, Avenida Pará 1720, Campus Umuarama, Bloco 2B, Bairro Umuarama, Uberlândia, MG, 38400-902, Brazil

    Paula Dechichi

  4. Department of Postgraduate Program in Dentistry, CEUMA University, São Luis, MA, Brazil

    Rafael Soares Diniz

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  1. Eduardo Moura Mendes
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  2. Milena Suemi Irie
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Correspondence to Priscilla Barbosa Ferreira Soares.

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Mendes, E.M., Irie, M.S., Rabelo, G.D. et al. Effects of ionizing radiation on woven bone: influence on the osteocyte lacunar network, collagen maturation, and microarchitecture. Clin Oral Invest 24, 2763–2771 (2020). https://doi.org/10.1007/s00784-019-03138-x

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