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Compromised inflammatory cytokine response to P. gingivalis LPS by fibroblasts from inflamed human gingiva

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Abstract

Objectives

The aims of this study were to compare the in vitro cytokine response of gingival fibroblasts (GF’s) from healthy and inflamed human gingival tissues and to assess whether GF’s from inflamed gingivae are capable of mounting a secondary inflammatory response after exposure to P. gingivalis LPS.

Materials and methods

GF’s were obtained from healthy donors and periodontitis patients and cultured in vitro. Cells were exposed to P. gingivalis LPS for 24h before measurement of MCP-1, GRO, IL-6, IL-8 and VEGF using a bead-based multiplex assay. Statistical comparisons were made between LPS-exposed GF’s and unstimulated cells as well as the two patient groups by two-way ANOVA.

Results

GF’s exposed to P. gingivalis LPS significantly increased their production of MCP-1, GRO, IL-6, IL-8 and VEGF compared to unstimulated cells. GF’s isolated from inflamed tissue from periodontitis patients demonstrated consistently less cytokine production after exposure to P. gingivalis LPS, most notably for GRO and IL-6.

Conclusions

The current study demonstrates that GF’s play an active role in the inflammatory response in periodontal disease by producing a number of chemokines and cytokines. Furthermore, inflamed GF’s may be compromised in their ability to mount an adequate secondary immune response in relation to chemokine/cytokine production.

Clinical relevance

The compromised inflammatory cytokine response of inflamed human gingival fibroblasts to P. gingivalis LPS may impact on their ability to recruit and activate inflammatory cells while maintaining persistent inflammation, a key feature of periodontal disease.

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Author information

Authors and Affiliations

  1. Adelaide Dental School, The University of Adelaide, North Terrace, Adelaide, SA, 5005, Australia

    Tracy R. Fitzsimmons & P. Mark Bartold

  2. Shandong Provincial Key Laboratory of Oral Tissue Regeneration Department of Periodontology, School of Stomatology, Shandong University, Shandong Province, Jinan, China

    Shaohua Ge

Authors
  1. Tracy R. Fitzsimmons
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  2. Shaohua Ge
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  3. P. Mark Bartold
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Corresponding author

Correspondence to Tracy R. Fitzsimmons.

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Conflict of interest

The authors declare that they have no competing interests.

Funding

This study was supported with internal funding from the University of Adelaide.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Fitzsimmons, T.R., Ge, S. & Bartold, P.M. Compromised inflammatory cytokine response to P. gingivalis LPS by fibroblasts from inflamed human gingiva. Clin Oral Invest 22, 919–927 (2018). https://doi.org/10.1007/s00784-017-2171-6

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  • DOI: https://doi.org/10.1007/s00784-017-2171-6

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