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Co-dependence of genotype and dietary protein intake to affect expression on amino acid/peptide transporters in porcine skeletal muscle

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Abstract

A total of 96 barrows (48 pure-bred Bama mini-pigs representing fatty genotype, and 48 Landrace pigs representing lean genotype) were randomly assigned to either a low- or adequate-protein treatment diet. The experimental period commenced at 5 weeks of age and extended to the finishing period. After euthanasia, blood and skeletal muscle samples were collected from pigs at the nursery, growing, and finishing phases. Our results indicate that the concentrations of free AAs in the plasma and muscle decreased as the age of the pigs increased. In addition, a strain × growth phase interaction (P < 0.05) was observed for the free AA pool in the plasma and muscle. The low-protein diet upregulated (P < 0.05) the mRNA levels for T1R1/T1R3 involved in glutamate binding, but downregulated (P < 0.05) the mRNA levels for PAT1, PAT2, and ASCT2, which transport neutral AAs into muscles. Bama mini-pigs had higher (P < 0.05) mRNA levels for LAT1, SNAT2, and EAAC1, but a lower (P < 0.05) mRNA level for PepT1, compared with Landrace pigs. Collectively, our findings indicate that adequate provision of dietary protein plays an important role in regulating profiles of free AA pools and expression of key AA/peptide transporters/transceptors in a genotype- and tissue-specific manner.

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Acknowledgments

The present work was jointly supported by grants from the National Basic Research Program of China (No. 2012CB124704 and 2013CB127305), National Nature Science Foundation of China (31372325, 31270044), K.C. Wong Education Foundation (Hong Kong), and Texas A&M AgriLife Research.

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Authors and Affiliations

  1. Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center of Healthy Livestock and Poultry, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China

    Y. Liu, X. Kong, F. Li, B. Tan, Y. Li, Y. Duan & Y. Yin

  2. Hunan Institute of Animal and Veterinary Sciences, Changsha, 410131, Hunan, China

    Y. Liu

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Y. Liu, Y. Li & Y. Duan

  4. College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, China

    J. He

  5. Department of Biochemistry and Molecular Biology, University of New Mexico, MSC08 4670, BMSB-258, 915 Camino de Salud, Albuquerque, NM, 87131-0001, USA

    C. Hu

  6. INRA, CNRH-IdF, AgroParisTech, UMR 914 Nutrition Physiology and Ingestive Behavior, 16 rue Claude Bernard, Paris, 75005, France

    F. Blachier

  7. Department of Animal Science, Texas A&M University, College Station, TX, 77843-2471, USA

    Guoyao Wu

  8. School of Biology, Hunan Normal Univesity, Changsha, Hunan, 410018, China

    Y. Yin

  9. Changsha Lvye Biotechnology Limited Company Academician Expert Workstation, Guangdong Wangda Group Academician Workstation for Clean Feed Technology Research and Development in Swine, Guangdong Hinapharm Group Academician Workstation for Biological Feed and Feed Additives and Animal Intestinal Health, Changsha, China

    Y. Yin

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  1. Y. Liu
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Correspondence to X. Kong or Y. Yin.

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Liu, Y., Kong, X., Li, F. et al. Co-dependence of genotype and dietary protein intake to affect expression on amino acid/peptide transporters in porcine skeletal muscle. Amino Acids 48, 75–90 (2016). https://doi.org/10.1007/s00726-015-2066-2

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  • DOI: https://doi.org/10.1007/s00726-015-2066-2

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