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Improved Sensitivity by Isotopic Substitution in Distance Measurements Based on Double Quantum Coherence EPR

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Abstract

We studied the effects of isotopic substitution (namely, deuterium substitution in solvents and 15N substitution in a spin label) on improving the sensitivity of distance measurements based on double quantum coherence (DQC) electron paramagnetic resonance (EPR) using cardiac troponin C. Deuteration of solvents increased the phase memory time, T m, by a factor of only 1.7, whereas the DQC amplitude increased significantly at long pulse intervals. The two-pulse echo amplitude was 1.7 times larger for the 15N spin label than it was for the 14N label because of the limited bandwidth of the microwave pulses. In the case of DQC, the echo is 2.7 times greater for the 15N label than it is for the 14N label due to the accumulated effects of the finite bandwidth. The combination of the two isotopic substitutions is expected to increase the sensitivity by about a factor of 6 when measuring distances longer than 4–5 nm.

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Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research (nos. 21023004 and 22350002) from the Ministry of Education, Science, Culture, and Sports (Japan) and by the Centers of Research Excellence in Science and Technology program of the Japanese Science and Technology Agency.

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Authors and Affiliations

  1. Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, 980-8577, Japan

    Jun Abe, Seigo Yamauchi & Yasunori Ohba

  2. Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Shido 1314-1, Samuki, Kagawa, 769-2193, Japan

    Shoji Ueki

  3. Department of Biological Sciences, Graduate School of Science, Osaka University, Machikaneyama 1-1, Toyonaka, Osaka, 560-0043, Japan

    Toshiaki Arata

  4. Department of Chemistry, Graduate School of Science, Osaka City University, 3-3-138 Sugimoto, Sumiyoshi, Osaka, 558-8585, Japan

    Sigeaki Nakazawa

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  1. Jun Abe
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  2. Shoji Ueki
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  3. Toshiaki Arata
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  4. Sigeaki Nakazawa
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Correspondence to Yasunori Ohba.

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Abe, J., Ueki, S., Arata, T. et al. Improved Sensitivity by Isotopic Substitution in Distance Measurements Based on Double Quantum Coherence EPR. Appl Magn Reson 42, 473–485 (2012). https://doi.org/10.1007/s00723-012-0317-x

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  • DOI: https://doi.org/10.1007/s00723-012-0317-x

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