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Introgression of the heterologous nuclear DNAs and efficacious compositions from Swertia tetraptera Maxim. into Bupleurum scorzonerifolium Willd. via somatic hybridization

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Abstract

Swertia tetraptera Maxim. is an important source of secoiridoid glucosides. To produce these pharmacologically valuable compounds heterologously in somatic hybrid cell lines, S. tetraptera protoplasts were irradiated with various doses of UV light and fused with protoplasts from a long-term cell line of Bupleurum scorzonerifolium Willd. This recipient was chosen as the cell line is cytogenetically stable and fast growing; furthermore, protoplasts isolated from the cell line are readily regenerable. From a set of 86 putative hybrid calli, only two were able to regenerate viable green plants. The hybridity of the 19 of the 86 selections was revealed by a combined isozyme and RAPD analysis, supported by a karyotypic study based on genomic in situ hybridization (GISH). Clone I-3 contained 0.014% swertiamarin while the regenerants had 0.069% swertiamarin and 0.409% gentiopicroside while the III-4 plants contained only 0.015% gentiopicroside.

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Abbreviations

GISH:

Genomic in situ hybridization

PEG:

Polyethylene glycol

RAPD:

Random amplified polymorphic DNA

UV:

Ultraviolet

2,4-D:

2,4-Dichlorophenoxyacetic acid

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Acknowledgements

This research was financially supported by the Chinese National Special Science Research Program (grant no. 2007CB948203), National Natural Science Foundation (grant nos. 30970243, 30771116 and 30470154) and Natural Education Ministry Doctor Station Foundation Fellowship (grant no. 913111006); and the Shandong Province Excellent Youth Foundation (grant no. JQ200810) and Science & Technology Plan (grant 2009GG10002001).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Anhui Medical University, Hefei, 230061, China

    Li Jiang

  2. Department of Biology, Qilu Normal University, Wenbo Lu 2#, Zhangqiu, 250200, China

    Yunfei Cai

  3. Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Shanda Nanlu 27#, Jinan, 250100, China

    Guangmin Xia & Fengning Xiang

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  1. Li Jiang
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Correspondence to Fengning Xiang.

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Handling Editor: Peter Nick

Li Jiang and Yunfei Cai contributed equally to this work.

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Jiang, L., Cai, Y., Xia, G. et al. Introgression of the heterologous nuclear DNAs and efficacious compositions from Swertia tetraptera Maxim. into Bupleurum scorzonerifolium Willd. via somatic hybridization. Protoplasma 249, 737–745 (2012). https://doi.org/10.1007/s00709-011-0317-y

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