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Effect of heat stress on actin cytoskeleton and endoplasmic reticulum of tobacco BY-2 cultured cells and its inhibition by Co2+

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Abstract

Temperature stress such as heat, cold, or freezing is a principal cause for yield reduction in crops. In particular, heat stress is very common and dangerous for plants since this stress can impact several plant and cellular functions. In spite of their role in sensing local stress and in controlling fundamental processes including PCD, the responses of cellular structures and organelles to heat stress are poorly investigated. In this work, we investigated the possible changes induced by mild heat stress, medium heat stress, and heat shock (HS; 5 min at 35°C, 45°C, or 50°C, respectively) on actin cytoskeleton and endoplasmic reticulum (ER) of tobacco BY-2 cultured cells. While mild and medium heat stresses are ineffective, HS induces depolymerization of actin microfilaments and changes in ER morphology accompanied by accumulation of the HSP70 binding protein (BiP). These effects of HS are prevented by the inhibitor of ethylene production Co2+. While the analyzed cell structures do not seem to be involved in the establishment of mild and medium heat stresses at least in this experimental system, the strong modifications induced by the treatment at 50°C suggest that actin cytoskeleton and ER may be involved in the responses to HS. Besides, the inhibiting effect of Co2+ suggests a role for ethylene as a regulative molecule in the responses to HS here observed.

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Abbreviations

BiP:

Binding protein

DiOC6 :

3,3’-Dihexyloxacarbocyanine iodide

ER:

Endoplasmic reticulum

HS:

Heat shock

PCD:

Programmed cell death

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Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy

    Massimo Malerba & Paolo Crosti

  2. Dipartimento di Scienze dell’Ambiente e del Territorio, Università degli Studi di Milano-Bicocca, Milan, Italy

    Raffaella Cerana

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  1. Massimo Malerba
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  2. Paolo Crosti
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  3. Raffaella Cerana
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Correspondence to Massimo Malerba.

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Malerba, M., Crosti, P. & Cerana, R. Effect of heat stress on actin cytoskeleton and endoplasmic reticulum of tobacco BY-2 cultured cells and its inhibition by Co2+ . Protoplasma 239, 23–30 (2010). https://doi.org/10.1007/s00709-009-0078-z

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  • DOI: https://doi.org/10.1007/s00709-009-0078-z

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