Skip to main content
SpringerLink
Log in

Cell-type-specific disruption and recovery of the cytoskeleton in Arabidopsis thaliana epidermal root cells upon heat shock stress

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary.

The cytoskeleton in plant cells plays an important role in controlling cell shape and mediating intracellular signalling. However, almost nothing is known about the reactions of cytoskeletal elements to heat stress, which represents one of the major environmental challenges for plants. Here we show that living epidermal root cells of Arabidopsis thaliana could cope with short-term heat shock stress showing disruption and subsequent recovery of microtubules and actin microfilaments in a time-dependent manner. Time-lapse imaging revealed a very dynamic behavior of both cytoskeletal elements including transient depolymerization and disassembly upon heat shock (40–41 °C) followed by full recovery at room temperature (20 °C) within 1–3 h. Reaction of microtubules, but not actin filaments, to heat shock was dependent on cell type and developmental stage. On the other hand, recovery of actin filaments, but not microtubules, from heat shock stress was dependent on the same parameters. The relevance of this adaptive cytoskeletal behavior to intracellular signalling is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

HSP:

heat shock protein

MAP:

microtubule-associated protein

MT:

microtubule

References

  • A Abdrakhamanova QY Wang L Khokhlova P Nick (2003) ArticleTitleIs microtubule disassembly a trigger for cold acclimation? Plant Cell Physiol 44 676–686 Occurrence Handle12881495 Occurrence Handle10.1093/pcp/pcg097 Occurrence Handle1:CAS:528:DC%2BD3sXlslGmur0%3D

    Article  PubMed  CAS  Google Scholar 

  • SK Baniwal K Bharti KY Chan M Fauth A Ganguli S Kotak SK Mishra L Nover M Port KD Scharf J Tripp C Weber D Zielinski P von Koskull-Doring (2004) ArticleTitleHeat stress response in plants: a complex game with chaperones and more than twenty heat stress transcription factors J Biosci 29 471–487 Occurrence Handle15625403 Occurrence Handle1:CAS:528:DC%2BD2MXhtFWrurg%3D

    PubMed  CAS  Google Scholar 

  • PW Barlow F Baluška (2000) ArticleTitleCytoskeletal perspectives on root growth and morphogenesis Annu Rev Plant Physiol Plant Mol Biol 51 289–322 Occurrence Handle15012194 Occurrence Handle10.1146/annurev.arplant.51.1.289 Occurrence Handle1:CAS:528:DC%2BD3cXlsVymtrc%3D

    Article  PubMed  CAS  Google Scholar 

  • J Chan CG Jensen LC Jensen M Bush CW Lloyd (1999) ArticleTitleThe 65-kDa carrot microtubule-associated protein forms regularly arranged filamentous cross-bridges between microtubules Proc Natl Acad Sci USA 96 14931–14936 Occurrence Handle10611315 Occurrence Handle10.1073/pnas.96.26.14931 Occurrence Handle1:CAS:528:DC%2BD3cXhtFamtw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • F Cvrckova F Rivero B Bavlnka (2004) ArticleTitleEvolutionarily conserved modules in actin nucleation: lessons from Dictyostelium discoideum and plants Protoplasma 224 15–31 Occurrence Handle15726806 Occurrence Handle1:STN:280:DC%2BD2M%2Fps1Cnug%3D%3D

    PubMed  CAS  Google Scholar 

  • A Desai TJ Mitchison (1997) ArticleTitleMicrotubule polymerization dynamics Annu Rev Cell Dev Biol 13 83–117 Occurrence Handle9442869 Occurrence Handle10.1146/annurev.cellbio.13.1.83 Occurrence Handle1:CAS:528:DyaK1cXisFSksg%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • P Dhonukshe AM Laxalt J Goedhart TW Gadella T Munnik (2003) ArticleTitlePhospholipase D activation correlates with microtubule reorganization in living plant cells Plant Cell 15 2666–2679 Occurrence Handle14508002 Occurrence Handle10.1105/tpc.014977 Occurrence Handle1:CAS:528:DC%2BD3sXpt1Orur0%3D

    Article  PubMed  CAS  Google Scholar 

  • P Dhonukshe J Mathur M Hülskamp TW Gadella SuffixJr (2005) ArticleTitleMicrotubule plus-ends reveal essential links between intracellular polarization and localized modulation of endocytosis during division-plane establishment in plant cells BMC Biol 3 11 Occurrence Handle15831100 Occurrence Handle10.1186/1741-7007-3-11

    Article  PubMed  Google Scholar 

  • CM Dreiza CM Brophy P Komalavilas EJ Furnish L Joshi MA Pallero JE Murphy-Ullrich M von Rechenberg YS Ho B Richardson N Xu Y Zhen JM Peltier A Panitch (2005) ArticleTitleTransducible heat shock protein 20 (HSP20) phosphopeptide alters cytoskeletal dynamics FASEB J 19 261–263 Occurrence Handle15598710 Occurrence Handle1:CAS:528:DC%2BD2MXhtFehs7o%3D

    PubMed  CAS  Google Scholar 

  • M Erhardt V Stoppin-Mellet S Campagne J Canaday J Mutterer T Fabian M Sauter T Muller C Peter AM Lambert AC Schmit (2002) ArticleTitleThe plant Spc98p homologue colocalizes with gamma-tubulin at microtubule nucleation sites and is required for microtubule nucleation J Cell Sci 115 2423–2431 Occurrence Handle12006626 Occurrence Handle1:CAS:528:DC%2BD38XltF2ksrg%3D

    PubMed  CAS  Google Scholar 

  • B Favery LA Chelysheva M Lebris F Jammes A Marmagne J De Almeida-Engler P Lecomte C Vaury RA Arkowitz P Abad (2004) ArticleTitle Arabidopsis formin AtFH6 is a plasma membrane-associated protein upregulated in giant cells induced by parasitic nematodes Plant Cell 16 2529–2540 Occurrence Handle15319477 Occurrence Handle10.1105/tpc.104.024372 Occurrence Handle1:CAS:528:DC%2BD2cXnvVarur8%3D

    Article  PubMed  CAS  Google Scholar 

  • MD Galigniana JM Harrell HM O’Hagen M Ljungman WB Pratt (2004) ArticleTitleHsp90-binding immunophilins link p53 to dynein during p53 transport to the nucleus J Biol Chem 279 22483–22489 Occurrence Handle15004035 Occurrence Handle10.1074/jbc.M402223200 Occurrence Handle1:CAS:528:DC%2BD2cXjvF2rtL8%3D

    Article  PubMed  CAS  Google Scholar 

  • CD Justus P Anderhag JL Goins MD Lazzaro (2004) ArticleTitleMicrotubules and microfilaments coordinate to direct a fountain streaming pattern in elongating conifer pollen tube tips Planta 219 103–109 Occurrence Handle14740215 Occurrence Handle10.1007/s00425-003-1193-2 Occurrence Handle1:CAS:528:DC%2BD2cXjs1eisL0%3D

    Article  PubMed  CAS  Google Scholar 

  • S Khawaja GG Gundersen JC Bulinski (1988) ArticleTitleEnhanced stability of microtubules enriched in detyrosinated tubulin is not a direct function of detyrosination level J Cell Biol 106 141–149 Occurrence Handle3276710 Occurrence Handle10.1083/jcb.106.1.141 Occurrence Handle1:CAS:528:DyaL1cXhtFSjsLY%3D

    Article  PubMed  CAS  Google Scholar 

  • LP Khokhlova OV Olinevich M Raudaskoski (2003) ArticleTitleReorganization of the microtubule and actin cytoskeleton in root cells of Triticum aestivum L. during low temperature and abscisic acid treatments Cell Biol Int 27 211–212 Occurrence Handle12681311 Occurrence Handle10.1016/S1065-6995(02)00336-0 Occurrence Handle1:CAS:528:DC%2BD3sXislCjsrk%3D

    Article  PubMed  CAS  Google Scholar 

  • G Komis P Apostolakos B Galatis (2002) ArticleTitleHyperosmotic stress-induced actin filament reorganization in leaf cells of Chlorophyton comosum J Exp Bot 53 1699–1710 Occurrence Handle12147720 Occurrence Handle10.1093/jxb/erf018 Occurrence Handle1:CAS:528:DC%2BD38Xmt12nsL4%3D

    Article  PubMed  CAS  Google Scholar 

  • TE Kreis (1987) ArticleTitleMicrotubules containing detyrosinated tubulin are less dynamic EMBO J 6 2597–2606 Occurrence Handle3315650 Occurrence Handle1:CAS:528:DyaL1cXjvVGn

    PubMed  CAS  Google Scholar 

  • V Link AK Sinha P Vashista MG Hofmann RK Proels R Ehness T Roitsch (2002) ArticleTitleA heat-activated MAP kinase in tomato: a possible regulator of the heat stress response FEBS Lett 531 179–183 Occurrence Handle12417308 Occurrence Handle10.1016/S0014-5793(02)03498-1 Occurrence Handle1:CAS:528:DC%2BD38Xotl2ntbY%3D

    Article  PubMed  CAS  Google Scholar 

  • J Marc CL Granger J Brincat DD Fisher T Kao AG McCubbin RJ Cyr (1998) ArticleTitleA GFP-MAP4 reporter gene for visualizing cortical microtubule rearrangements in living epidermal cells Plant Cell 10 1927–1940 Occurrence Handle9811799 Occurrence Handle10.1105/tpc.10.11.1927 Occurrence Handle1:CAS:528:DyaK1MXptFSk

    Article  PubMed  CAS  Google Scholar 

  • J Mathur (2004) ArticleTitleCell shape development in plants Trends Plant Sci 9 583–590 Occurrence Handle15564125 Occurrence Handle10.1016/j.tplants.2004.10.006 Occurrence Handle1:CAS:528:DC%2BD2cXhtVaksLrK

    Article  PubMed  CAS  Google Scholar 

  • J Mathur M Hulskamp (2002) ArticleTitleMicrotubules and microfilaments in cell morphogenesis in higher plants Curr Biol 12 R669–R676 Occurrence Handle12361589 Occurrence Handle10.1016/S0960-9822(02)01164-8 Occurrence Handle1:CAS:528:DC%2BD38Xns1Sht7g%3D

    Article  PubMed  CAS  Google Scholar 

  • C Mazars L Thion P Thuleau A Graziana MR Knight M Moreau R Ranjeva (1997) ArticleTitleOrganization of cytoskeleton controls the changes in cytosolic calcium of cold-shocked Nicotiana plumbaginifolia protoplasts Cell Calcium 22 413–420 Occurrence Handle9448947 Occurrence Handle10.1016/S0143-4160(97)90025-7 Occurrence Handle1:CAS:528:DyaK1cXhsl2qtw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • DW McCurdy DR Kovar CJ Staiger (2001) ArticleTitleActin and actin-binding proteins in higher plants Protoplasma 215 89–104 Occurrence Handle11732068 Occurrence Handle10.1007/BF01280306 Occurrence Handle1:CAS:528:DC%2BD3MXltVKrtbs%3D

    Article  PubMed  CAS  Google Scholar 

  • A Michelot C Guerin S Huang M Ingouff S Richard N Rodiuc CJ Staiger L Blanchoin (2005) ArticleTitleThe formin homology 1 domain modulates the actin nucleation and bundling activity of Arabidopsis FORMIN1 Plant Cell 17 2296–2313 Occurrence Handle15994911 Occurrence Handle10.1105/tpc.105.030908 Occurrence Handle1:CAS:528:DC%2BD2MXpsFGjsLg%3D

    Article  PubMed  CAS  Google Scholar 

  • T Mitchison M Kirschner (1984) ArticleTitleDynamic instability of microtubule growth Nature 312 237–242 Occurrence Handle6504138 Occurrence Handle10.1038/312237a0 Occurrence Handle1:CAS:528:DyaL2MXhvF2jsQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • BL Orvar V Sangwan F Omann RS Dhindsa (2000) ArticleTitleEarly steps in cold sensing by plant cells: the role of actin cytoskeleton and membrane fluidity Plant J 23 785–794 Occurrence Handle10998189 Occurrence Handle10.1046/j.1365-313x.2000.00845.x Occurrence Handle1:CAS:528:DC%2BD3cXnslWgt7o%3D

    Article  PubMed  CAS  Google Scholar 

  • E Panteris B Galatis (2005) ArticleTitleThe morphogenesis of lobed plant cells in the mesophyll and epidermis: organization and distinct roles of cortical microtubules and actin filaments New Phytol 167 721–732 Occurrence Handle16101909 Occurrence Handle10.1111/j.1469-8137.2005.01464.x Occurrence Handle1:CAS:528:DC%2BD2MXhtVGisbfL

    Article  PubMed  CAS  Google Scholar 

  • ML Preuss DR Kovar YR Lee CJ Staiger DP Delmer B Liu (2004) ArticleTitleA plant-specific kinesin binds to actin microfilaments and interacts with cortical microtubules in cotton fibers Plant Physiol 136 3945–3955 Occurrence Handle15557092 Occurrence Handle10.1104/pp.104.052340 Occurrence Handle1:CAS:528:DC%2BD2MXjtVamuw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • R Saedler N Mathur BP Srinivas B Kernebeck M Hulskamp J Mathur (2004) ArticleTitleActin control over microtubules suggested by DISTORTED2 encoding the Arabidopsis ARPC2 subunit homolog Plant Cell Physiol 45 813–822 Occurrence Handle15295064 Occurrence Handle10.1093/pcp/pch103 Occurrence Handle1:CAS:528:DC%2BD2cXmsFeqsLY%3D

    Article  PubMed  CAS  Google Scholar 

  • J Šamaj M Peters D Volkmann F Baluška (2000) ArticleTitleEffects of myosin AT-Pase inhibitor 2,3-butanedione 2-monoxime on distributions of myosins, F-actin, microtubules, and cortical endoplasmic reticulum in maize root apices Plant Cell Physiol 41 571–582 Occurrence Handle10929940

    PubMed  Google Scholar 

  • J Šamaj M Ovecka A Hlavacka F Lecourieux I Meskiene I Lichtscheidl P Lenart J Salaj D Volkmann L Bogre F Baluška H Hirt (2002) ArticleTitleInvolvement of the mitogen-activated protein kinase SIMK in regulation of root hair tip growth EMBO J 21 3296–3306 Occurrence Handle12093731 Occurrence Handle10.1093/emboj/cdf349

    Article  PubMed  Google Scholar 

  • J Šamaj F Baluška H Hirt (2004a) ArticleTitleFrom signal to cell polarity: mitogen-activated protein kinases as sensors and effectors of cytoskeleton dynamicity J Exp Bot 55 189–198

    Google Scholar 

  • J Šamaj F Baluška B Voigt M Schlicht D Volkmann D Menzel (2004b) ArticleTitleEndocytosis, actin cytoskeleton and signalling Plant Physiol 135 1150–1161 Occurrence Handle10.1104/pp.104.040683

    Article  Google Scholar 

  • J Šamaj ND Read D Volkmann D Menzel F Baluška (2005) ArticleTitleThe endocytic network in higher plants Trends Cell Biol 15 425–433 Occurrence Handle16006126 Occurrence Handle10.1016/j.tcb.2005.06.006

    Article  PubMed  Google Scholar 

  • J Šamaj F Baluška B Voigt D Volkmann D Menzel (2006) Endocytosis and actin cytoskeleton in plant cell morphogenesis J Šamaj F Baluška D Menzel (Eds) The plant endocytosis Springer Berlin Heidelberg New York 233–244

    Google Scholar 

  • V Sangwan BL Orvar J Beyerly H Hirt RS Dhindsa (2002) ArticleTitleOpposite changes in membrane fluidity mimic cold and heat stress activation of distinct plant MAP kinase pathways Plant J 31 629–638 Occurrence Handle12207652 Occurrence Handle10.1046/j.1365-313X.2002.01384.x Occurrence Handle1:CAS:528:DC%2BD38Xnsl2hurs%3D

    Article  PubMed  CAS  Google Scholar 

  • JC Sedbrook (2004) ArticleTitleMAPs in plant cells: delineating microtubule growth dynamics and organization Curr Opin Plant Biol 7 632–640 Occurrence Handle15491911 Occurrence Handle10.1016/j.pbi.2004.09.017 Occurrence Handle1:CAS:528:DC%2BD2cXos1ChsLc%3D

    Article  PubMed  CAS  Google Scholar 

  • SL Shaw R Kamyar DW Ehrhardt (2003) ArticleTitleSustained microtubule treadmilling in Arabidopsis cortical arrays Science 300 1715–1718 Occurrence Handle12714675 Occurrence Handle10.1126/science.1083529 Occurrence Handle1:CAS:528:DC%2BD3sXksVKis70%3D

    Article  PubMed  CAS  Google Scholar 

  • T Shimmen E Yokota (2004) ArticleTitleCytoplasmic streaming in plants Curr Opin Cell Biol 16 68–72 Occurrence Handle15037307 Occurrence Handle10.1016/j.ceb.2003.11.009 Occurrence Handle1:CAS:528:DC%2BD2cXitlSms7s%3D

    Article  PubMed  CAS  Google Scholar 

  • BJ Sieberer T Ketelaar JJ Esseling AM Emons (2005) ArticleTitleMicrotubules guide root hair tip growth New Phytol 167 711–719 Occurrence Handle16101908 Occurrence Handle10.1111/j.1469-8137.2005.01506.x Occurrence Handle1:CAS:528:DC%2BD2MXhtVGisbfK

    Article  PubMed  CAS  Google Scholar 

  • M Sivaguru F Baluška D Volkmann HH Felle WJ Horst (1999) ArticleTitleImpacts of aluminum on the cytoskeleton of the maize root apex. short-term effects on the distal part of the transition zone Plant Physiol 119 1073–1082 Occurrence Handle10069846 Occurrence Handle10.1104/pp.119.3.1073 Occurrence Handle1:CAS:528:DyaK1MXhvFymurc%3D

    Article  PubMed  CAS  Google Scholar 

  • M Sivaguru S Pike W Gassmann TI Baskin (2003) ArticleTitleAluminum rapidly depolymerizes cortical microtubules and depolarizes the plasma membrane: evidence that these responses are mediated by a glutamate receptor Plant Cell Physiol 44 667–675 Occurrence Handle12881494 Occurrence Handle10.1093/pcp/pcg094 Occurrence Handle1:CAS:528:DC%2BD3sXlslGmurw%3D

    Article  PubMed  CAS  Google Scholar 

  • A Smertenko P Dráber V Viklický Z Opatrný (1997) ArticleTitleHeat stress affects the organization of microtubules and cell division in Nicotiana tabacum cells Plant Cell Environ 20 1534–1542 Occurrence Handle10.1046/j.1365-3040.1997.d01-44.x

    Article  Google Scholar 

  • A Smertenko N Saleh H Igarashi H Mori I Hauser-Hahn CJ Jiang S Sonobe CW Lloyd PJ Hussey (2000) ArticleTitleA new class of microtubule-associated proteins in plants Nat Cell Biol 2 750–753 Occurrence Handle11025667 Occurrence Handle10.1038/35036390 Occurrence Handle1:CAS:528:DC%2BD3cXnsVyntLc%3D

    Article  PubMed  CAS  Google Scholar 

  • AP Smertenko PV Bozhkov LH Filonova S von Arnold PJ Hussey (2003) ArticleTitleRe-organisation of the cytoskeleton during developmental programmed cell death in Picea abies embryos Plant J 33 813–824 Occurrence Handle12609024 Occurrence Handle10.1046/j.1365-313X.2003.01670.x Occurrence Handle1:CAS:528:DC%2BD3sXis1Gls7o%3D

    Article  PubMed  CAS  Google Scholar 

  • LG Smith DG Oppenheimer (2005) ArticleTitleSpatial control of cell expansion by the plant cytoskeleton Annu Rev Cell Dev Biol 21 271–295 Occurrence Handle16212496 Occurrence Handle10.1146/annurev.cellbio.21.122303.114901 Occurrence Handle1:CAS:528:DC%2BD2MXhtlektbrJ

    Article  PubMed  CAS  Google Scholar 

  • CJ Staiger (2000) ArticleTitleSignaling to the actin cytoskeleton in plants Annu Rev Plant Physiol Plant Mol Biol 51 257–288 Occurrence Handle15012193 Occurrence Handle10.1146/annurev.arplant.51.1.257 Occurrence Handle1:CAS:528:DC%2BD3cXlsVymtrY%3D

    Article  PubMed  CAS  Google Scholar 

  • CJ Staiger VE Franklin-Tong (2003) ArticleTitleThe actin cytoskeleton is a target of the self-incompatibility response in Papaver rhoeas J Exp Bot 54 103–113 Occurrence Handle12456760 Occurrence Handle10.1093/jxb/54.380.103 Occurrence Handle1:CAS:528:DC%2BD3sXlt1Kkug%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • CJ Staiger PJ Hussey (2004) Actin and actin modulating proteins PJ Hussey (Eds) The plant cytoskeleton in cell differentiation and development Blackwell Oxford 32–80

    Google Scholar 

  • SG Thomas VE Franklin-Tong (2004) ArticleTitleSelf-incompatibility triggers programmed cell death in Papaver pollen Nature 429 305–309 Occurrence Handle15152254 Occurrence Handle10.1038/nature02540 Occurrence Handle1:CAS:528:DC%2BD2cXktVOquro%3D

    Article  PubMed  CAS  Google Scholar 

  • N Van Bruaene G Joss P Van Oostveldt (2004) ArticleTitleReorganization and in vivo dynamics of microtubules during Arabidopsis root hair development Plant Physiol 136 3905–3919 Occurrence Handle15557102 Occurrence Handle10.1104/pp.103.031591 Occurrence Handle1:CAS:528:DC%2BD2MXjtVamtA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • B Voigt AC Timmers J Šamaj J Müller F Baluska D Menzel (2005) ArticleTitleGFP-FABD2 fusion construct allows in vivo visualization of the dynamic actin cytoskeleton in all cells of Arabidopsis seedlings Eur J Cell Biol 84 595–608 Occurrence Handle16032928 Occurrence Handle10.1016/j.ejcb.2004.11.011 Occurrence Handle1:CAS:528:DC%2BD2MXotFeju7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • D Volkmann F Baluška (1999) ArticleTitleActin cytoskeleton in plants: from transport networks to signaling networks Microsc Res Tech 47 135–154 Occurrence Handle10523792 Occurrence Handle10.1002/(SICI)1097-0029(19991015)47:2<135::AID-JEMT6>3.0.CO;2-1 Occurrence Handle1:STN:280:DyaK1MvlsVOluw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • QY Wang P Nick (2001) ArticleTitleCold acclimation can induce microtubular cold stability in a manner distinct from abscisic acid Plant Cell Physiol 42 999–1005 Occurrence Handle11577195 Occurrence Handle10.1093/pcp/pce135 Occurrence Handle1:CAS:528:DC%2BD3MXntFWksb4%3D

    Article  PubMed  CAS  Google Scholar 

  • GO Wasteneys (2004) ArticleTitleProgress in understanding the role of microtubules in plant cells Curr Opin Plant Biol 7 651–660 Occurrence Handle15491913 Occurrence Handle10.1016/j.pbi.2004.09.008 Occurrence Handle1:CAS:528:DC%2BD2cXos1Chsb0%3D

    Article  PubMed  CAS  Google Scholar 

  • AT Whittington O Vugrek KJ Wei NG Hasenbein K Sugimoto MC Rashbrooke GO Wasteneys (2001) ArticleTitleMOR1 is essential for organizing cortical microtubules in plants Nature 411 610–613 Occurrence Handle11385579 Occurrence Handle10.1038/35079128 Occurrence Handle1:CAS:528:DC%2BD3MXksVSgsr4%3D

    Article  PubMed  CAS  Google Scholar 

  • B Wiesler QY Wang P Nick (2002) ArticleTitleThe stability of cortical microtubules depends on their orientation Plant J 32 1023–1032 Occurrence Handle12492843 Occurrence Handle10.1046/j.1365-313X.2002.01489.x Occurrence Handle1:CAS:528:DC%2BD3sXotFeqtw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • A Yoneda M Akatsuka F Kumagai S Hasezawa (2004) ArticleTitleDisruption of actin microfilaments causes cortical microtubule disorganization and extra-phragmoplast formation at M/G1 interface in synchronized tobacco cells Plant Cell Physiol 45 761–769 Occurrence Handle15215511 Occurrence Handle10.1093/pcp/pch091 Occurrence Handle1:CAS:528:DC%2BD2cXltlWrur8%3D

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Cellular and Molecular Botany, University of Bonn, Bonn

    J. Müller, D. Menzel & J. Šamaj

  2. Institute of Plant Genetics and Biotechnology, Slovak Academy of Sciences, Nitra

    J. Šamaj

Authors
  1. J. Müller
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. Menzel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Šamaj
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. Šamaj.

Additional information

Correspondence and reprints: Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, 53115 Bonn, Federal Republic of Germany.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Müller, J., Menzel, D. & Šamaj, J. Cell-type-specific disruption and recovery of the cytoskeleton in Arabidopsis thaliana epidermal root cells upon heat shock stress. Protoplasma 230, 231–242 (2007). https://doi.org/10.1007/s00709-006-0239-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00709-006-0239-2

Search

Navigation