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Complete genome sequence of camellia virus A, a tentative new member of the genus Waikavirus

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Abstract

A new positive-strand RNA virus genome was discovered in Camellia japonica plants. The complete genome of the virus is 12,570 nt in size, excluding the poly(A) tail, and contains one large open reading frame (ORF1) and two small open reading frames (ORF2, ORF3). ORF1 and ORF2 are homologous to sequences of waikaviruses, while ORF3 has no relatives in the databases. ORF1 encodes a putative polyprotein precursor that is putatively processed into eight smaller proteins, as in typical waikaviruses. Comprehensive analysis, including BLAST searches, genome organization and pairwise sequence comparisons, and phylogeny reconstructions, invariably placed the virus with the waikaviruses. Furthermore, due to lower amino acid sequence identity to known waikaviruses than the threshold species demarcation cutoff, this virus may represent a new species in the genus Waikavirus, family Secoviridae, and we have tentatively named it “camellia virus A” (CamVA). Finally, a field survey was conducted to assess the occurrence of CamVA in camellias and its associated symptoms.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (32072389), Innovation Program for Chongqing’s Overseas Returnees (cx2019013), and the 111 Project (B18044).

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Authors and Affiliations

  1. National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China

    Ruiling Liao, Qingying Chen, Song Zhang, Mengji Cao & Changyong Zhou

  2. Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China

    Ruiling Liao, Qingying Chen, Song Zhang, Mengji Cao & Changyong Zhou

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  1. Ruiling Liao
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  2. Qingying Chen
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  3. Song Zhang
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  4. Mengji Cao
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  5. Changyong Zhou
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Correspondence to Mengji Cao or Changyong Zhou.

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Liao, R., Chen, Q., Zhang, S. et al. Complete genome sequence of camellia virus A, a tentative new member of the genus Waikavirus. Arch Virol 166, 3207–3210 (2021). https://doi.org/10.1007/s00705-021-05216-6

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  • DOI: https://doi.org/10.1007/s00705-021-05216-6

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