Skip to main content

Advertisement

SpringerLink
Log in

Complete genome sequence of Edgeworthia chrysantha mosaic-associated virus, a tentative new member of the genus Coguvirus (family Phenuiviridae)

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

A new negative-strand RNA (nsRNA) virus genome was discovered in Edgeworthia chrysantha Lindl. This virus, tentatively named “Edgeworthia chrysantha mosaic-associated virus” (ECMaV), has a bipartite genome that comprises (i) a nsRNA1, encoding the viral RNA-dependent RNA polymerase (RdRp), and (ii) an ambisense RNA2, coding for the putative movement protein (MP) and nucleocapsid protein (NP), with the open reading frames separated by a long AU-rich intergenic region (IR). Sequence comparisons and phylogenetic analysis showed that the RdRp is closely related to those of other recently discovered plant-infecting nsRNA viruses in the new genus Coguvirus and that ECMaV can be classified as a member of a novel species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Kormelink R, Garcia ML, Goodin M et al (2011) Negative-strand RNA viruses: the plant-infecting counterparts. Virus Res 162:184–202

    Article  CAS  Google Scholar 

  2. Elliott RM, Brennan B (2014) Emerging phleboviruses. Curr Opin Virol 5:50–57

    Article  Google Scholar 

  3. Hubálek Z, Rudolf I, Nowotny N (2014) Arboviruses pathogenic for domestic and wild animals. Adv Virus Res 89:201–275

    Article  Google Scholar 

  4. Abudurexiti A, Adkins S, Alioto D, Alkhovsky S et al (2019) Taxonomy of the order Bunyavirales: Update 2019. Arch Virol 164:1949–1965

    Article  CAS  Google Scholar 

  5. Kuhn JH, Adkins S, Alioto D et al (2020) 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Arch Virol 165:3023–3072

    Article  Google Scholar 

  6. Junglen S (2016) Evolutionary origin of pathogenic arthropod-borne viruses—a case study in the family Bunyaviridae. Curr Opin Insect Sci 16:81–86

    Article  Google Scholar 

  7. Minutolo M, Cinque M, Chiumenti M, Serio FD, Alioto D, Navarro B (2021) Identification and characterization of citrus concave gum-associated virus infecting citrus and apple trees by serological, molecular and high-throughput sequencing approaches. Plants 10(11):2390

    Article  Google Scholar 

  8. Rott ME, Kesanakurti P et al (2018) Discovery of negative-sense RNA viruses in trees infected with apple rubbery wood disease by next-generation sequencing. Plant Dis 102(7):1254–1263

    Article  CAS  Google Scholar 

  9. Wang Y, Wang GP, Hong N et al (2019) First report of apple rubbery wood virus 2 infecting pear (Pyrus spp.) in China. Plant Dis 103(12):3293

    Article  Google Scholar 

  10. Navarro B, Zicca S, Minutolo M, Saponari M, Alioto D, Serio FD (2018) A negative-stranded RNA virus infecting citrus trees: the second member of a new genus within the order Bunyavirales. Front Microbiol 9:2340

    Article  Google Scholar 

  11. Flora of China Editorial Committee of Chinese Academy of Sciences (2004) The flora of China. Science Press: Beijing, China 52(1):389

  12. Cardin L, Moury B (2009) First report of cucumber mosaic virus in Edgeworthia chrysantha in France and Italy. J Plant Pathol 91(2):502

    Google Scholar 

  13. Haas BJ, Papanicolaou A, Yassour M et al (2013) De novo transcript sequence reconstruction from RNA-seq using the trinity platform for reference generation and analysis. Nat Protoc 8:1494–1512

    Article  CAS  Google Scholar 

  14. Standley D (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780

    Article  Google Scholar 

  15. Sudhir K, Glen S, Li M, Christina K, Koichiro T (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549

    Article  Google Scholar 

  16. Zhang S, Tian X, Navarro B, Serio FD, Cao MJ (2021) Watermelon crinkle leaf-associated virus 1 and watermelon crinkle leaf-associated virus 2 have a bipartite genome with molecular signatures typical of the members of the genus Coguvirus (family Phenuiviridae). Arch Virol 166:2829–2834

    Article  CAS  Google Scholar 

  17. Nguyen M, Haenni AL (2003) Expression strategies of ambisense viruses. Virus Res 93:141–150

    Article  CAS  Google Scholar 

  18. Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3415

    Article  CAS  Google Scholar 

  19. Bouloy M (2011) Bunyaviridae: molecular and cellular biology. In: Plyusnin A, Elliott RM (eds) Molecular biology of phleboviruses. Academic Press, Cambridge, pp 95–128

    Google Scholar 

  20. Navarro B, Maria M, Angelo DS, Francesco P, Daniela A, Francesco DS (2018) The first phlebo-like virus infecting plants: a case study on the adaptation of negative-stranded RNA viruses to new hosts. Mol Plant Patho 19(5):1075–1089

    Article  CAS  Google Scholar 

  21. Xin M, Cao MJ, Liu WW, Ren YD, Zhou XP, Wang XF (2017) Two negative-strand RNA viruses identified in watermelon represent a novel clade in the order Bunyavirales. Front Microbiol 8:1514

    Article  Google Scholar 

  22. Chen ZL, Anane RF, Wang Z et al (2021) Complete genome sequence analysis of a novel coguvirus isolated from Paris polyphylla var. yunnanensis. Arch Virol 166:2045–2050

    Article  CAS  Google Scholar 

  23. Tang LG, Song LP, Ye ZB et al (2021) A novel negative-stranded RNA virus of the order Bunyavirales identified in Brassica campestris L. ssp. chinensis. Arch Virol 166:1525–1528

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the National Natural Science Foundation of China (32072389), Innovation Program for Chongqing's Overseas Returnees (cx2019013), and 111 project (B18044).

Author information

Authors and Affiliations

  1. National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China

    Xiaoru Wang, Ruiling Liao, Xinying Yang, Qiyan Liu, Song Zhang & Mengji Cao

  2. Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China

    Xiaoru Wang, Ruiling Liao, Xinying Yang, Qiyan Liu, Song Zhang & Mengji Cao

Authors
  1. Xiaoru Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ruiling Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xinying Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qiyan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Song Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mengji Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mengji Cao.

Ethics declarations

Conflict of interest

All authors declare they have no conflict of interest.

Compliance with ethical standards

I have read and abided by the statement of ethical standards for manuscripts submitted to Archives of Virology.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling Editor: Sead Sabanadzovic.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (TXT 9 KB)

Rights and permissions

Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, X., Liao, R., Yang, X. et al. Complete genome sequence of Edgeworthia chrysantha mosaic-associated virus, a tentative new member of the genus Coguvirus (family Phenuiviridae). Arch Virol 167, 2827–2831 (2022). https://doi.org/10.1007/s00705-022-05608-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-022-05608-2

Search

Navigation