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Camellia ringspot-associated virus 4, a proposed new foveavirus from Camellia japonica

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Abstract

One large contig with high sequence similarity to Asian prunus virus 2 was identified by high-throughput sequencing from a camellia (Camellia japonica) tree with ringspot symptoms. The complete genome of this new virus was determined to be 8829 nucleotides long, excluding the 3′ poly(A) tail. Its genome organization resembles that of known foveaviruses but contains an additional open reading frame in the 3′-terminal region. Phylogenetic analysis also places this virus with members of the genus Foveavirus in the family Betaflexiviridae in the same subgroup. The virus, which is provisionally named "camellia ringspot-associated virus 4″, shares 50–56% nucleotide sequence identity with other foveaviruses and should represent a new species in the genus.

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Acknowledgements

The authors want to thank Erin Rao and Sam Grinstead for excellent technical support, Phil Normandy for providing garden samples, and Dr. Gary Kinard for critical review of the manuscript. This work was supported by the U.S. Department of Agriculture, Agriculture Research Service, under research project 1215-22000-302-00D “Characterizing and Detecting Pathogens to Ensure Safe Exchange of Plant Germplasm” and a Fujian province scholarship for overseas studies (Grant No. 2016-29).

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Authors and Affiliations

  1. USDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA

    Luping Zheng, Madeleine Chen & Ruhui Li

  2. College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Luping Zheng

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  1. Luping Zheng
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  2. Madeleine Chen
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  3. Ruhui Li
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Correspondence to Ruhui Li.

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Zheng, L., Chen, M. & Li, R. Camellia ringspot-associated virus 4, a proposed new foveavirus from Camellia japonica. Arch Virol 165, 1707–1710 (2020). https://doi.org/10.1007/s00705-020-04655-x

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  • DOI: https://doi.org/10.1007/s00705-020-04655-x

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