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A novel recombinant genome of minute virus of canines in China

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Abstract

In this study, we determined almost all of the genome sequence of minute virus of canines (MVC) strain CDK47/2017 and performed phylogenetic analysis with this isolate and other MVC isolates. The genome of CDK47/2017 has the following characteristics: 1) The amino acid sequence of the NS1 protein is similar to that of the novel strain 15D009/KT241026.1, which has 17 identical amino acid changes and two identical amino acid insertions compared with other known MVC strains. These two strains clustered in a unique branch in an NSI-based phylogenetic tree. 2) Phylogenetic analysis based on the NP1 protein showed that strain CDK47/2017clustered in an independent branch with strains 15D009/KT241026.1 and HM-6/AB158475.1, both of which has 10 identical amino acid changes compared with other known MVC strains. 3) Eight unique amino acid substitutions of the CDK47/2017 capsid protein resulted in it forming a unique branch in the phylogenetic tree. 4) Recombination events were identified between the 3′ end of the NS1 gene and 5′ end of NP1 gene. Together, these characteristics suggest that strain CDK47/2017 represents a novel MVC strain that is distinct from all known MVC strains with sequences in the GenBank database. This contributes to a greater understanding of the genetic evolution of MVC.

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Acknowledgements

This work was funded by the National Key Research and Development Program of China (grant number 2017YFD0501101), the Innovation Team for Animal Epidemic Diseases Prevention State Ethnic Affairs Commission (grant number 13TD0057), and the Innovative Research Project of Graduate Students in Southwest University for Nationalities (grant number CX2017SZ056).

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Authors and Affiliations

  1. College of Life Science and Technology, Southwest University for Nationalities, No. 16, South 4th Section 1st Ring Road, Chengdu, 610041, China

    Nan Yan, Hua Yue, Ruici Kan, Fan Feng & Cheng Tang

  2. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, China

    Hua Yue & Cheng Tang

Authors
  1. Nan Yan
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  2. Hua Yue
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  3. Ruici Kan
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  4. Fan Feng
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  5. Cheng Tang
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Corresponding author

Correspondence to Cheng Tang.

Ethics declarations

This study did not involve animal experiments other than collecting fecal samples from diarrheic dogs that were brought to animal hospitals for clinical treatment.

Conflict of interest

The authors declare that they have no conflicts of interest.

Additional information

Handling Editor: Sheela Ramamoorthy.

The genome sequence of MVC CDK47/2017 strain has been deposited in GenBank under accession no. MH051156.

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Yan, N., Yue, H., Kan, R. et al. A novel recombinant genome of minute virus of canines in China. Arch Virol 164, 861–865 (2019). https://doi.org/10.1007/s00705-018-4104-3

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  • DOI: https://doi.org/10.1007/s00705-018-4104-3

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