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Sequence analysis of an isolate of minute virus of canines in China reveals the closed association with bocavirus

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Abstract

In the present study, we have cloned and sequenced the nearly-full-length genome of minute virus of canines (MVC), SH26, in China. The genome of MVC, 5,132 nucleotides (nts) in length, contains three open reading frames (ORFs), which are 2,325-bp of NS1, 561-bp of NP1 and 2,112-bp of VP1/VP2 encoding three proteins of 774, 186 and 703 residues, respectively. Predicted amino acids sequence of NS1 of MVC has 44% identity with human bocavirus (HBoV) and human boacvirus 2 (HBoV2), NP1 has 48 and 45% identity with HBoV and HBoV2, VP1/VP2 has 45 and 46% identity with HBoV and HBoV2, respectively. Phylogenetic analysis showed that the present Chinese MVC strain was also closely clustered with the previous American and Japanese MVC isolates, and MVCs formed a different branch together with bovine parvovirus and HBoVs from other parvoviruses classified into Parvovirinae.

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Authors and Affiliations

  1. Zoonosis and Comparative Medicine Group, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, People’s Republic of China

    T. L. Shan, L. Cui, X. Q. Dai, W. Guo, X. G. Shang, Y. Yu, W. Zhang, Y. J. Kang, Q. Shen, Z. B. Yang, J. G. Zhu & X. G. Hua

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  1. T. L. Shan
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  2. L. Cui
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  3. X. Q. Dai
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  4. W. Guo
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  5. X. G. Shang
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  6. Y. Yu
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  7. W. Zhang
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  8. Y. J. Kang
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  9. Q. Shen
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  10. Z. B. Yang
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  11. J. G. Zhu
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  12. X. G. Hua
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Corresponding author

Correspondence to X. G. Hua.

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T. L. Shan and L. Cui contributed equally to this work.

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Shan, T.L., Cui, L., Dai, X.Q. et al. Sequence analysis of an isolate of minute virus of canines in China reveals the closed association with bocavirus. Mol Biol Rep 37, 2817–2820 (2010). https://doi.org/10.1007/s11033-009-9831-9

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  • DOI: https://doi.org/10.1007/s11033-009-9831-9

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