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Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana

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Abstract

Beauveria bassiana is a ubiquitous entomopathogen infecting hundreds of insect species. We have determined the genomic organization and the complete nucleotide sequence of a novel virus isolated from the isolate A24 of B. bassiana. Phylogenetic analysis of the polymerase gene reveals that the virus, tentatively named Beauveria bassiana virus 1, belongs to the family Amalgaviridae and represents a distinct lineage of amalgaviruses infecting fungi.

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References

  1. Nibert ML, Ghabrial SA, Maiss E et al (2014) Taxonomic reorganization of family Partitiviridae and other recent progress in partitivirus research. Virus Res 188C:128–141. doi:10.1016/j.virusres.2014.04.007

    Article  Google Scholar 

  2. Xiao G, Ying S-H, Zheng P et al (2012) Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana. Sci Rep 2:483. doi:10.1038/srep00483

    PubMed Central  PubMed  Google Scholar 

  3. Herrero N, Dueñas E, Quesada-Moraga E, Zabalgogeazcoa I (2012) Prevalence and diversity of viruses in the entomopathogenic fungus Beauveria bassiana. Appl Environ Microbiol 78:8523–8530. doi:10.1128/AEM.01954-12

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Yie SW, Khalifa ME, Hahn T, Pearson MN (2013) Molecular characterization of a novel victorivirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 159:1321–1327. doi:10.1007/s00705-013-1938-6

    Article  PubMed  Google Scholar 

  5. Castillo A, Cottet L, Castro M, Sepúlveda F (2011) Rapid isolation of mycoviral double-stranded RNA from Botrytis cinerea and Saccharomyces cerevisiae. Virol J 8:38. doi:10.1186/1743-422X-8-38

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Darissa O, Willingmann P, Adam G (2010) Optimized approaches for the sequence determination of double-stranded RNA templates. J Virol Methods 169:397–403. doi:10.1016/j.jviromet.2010.08.013

    Article  CAS  PubMed  Google Scholar 

  7. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols. A guide to methods and applications. Academic Press, New York, pp 315–322

    Chapter  Google Scholar 

  8. Lin Y, Zhang H, Zhao C et al (2014) The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28. Arch Virol. doi:10.1007/s00705-014-2218-9

    PubMed Central  Google Scholar 

  9. Sabanadzovic S, Abou Ghanem-Sabanadzovic N, Valverde RA (2010) A novel monopartite dsRNA virus from rhododendron. Arch Virol 155:1859–1863. doi:10.1007/s00705-010-0770-5

    Article  CAS  PubMed  Google Scholar 

  10. Sabanadzovic S, Valverde RA, Brown JK et al (2009) Southern tomato virus: the link between the families Totiviridae and Partitiviridae. Virus Res 140:130–137. doi:10.1016/j.virusres.2008.11.018

    Article  CAS  PubMed  Google Scholar 

  11. Liu W, Chen J (2009) A double-stranded RNA as the genome of a potential virus infecting Vicia faba. Virus Genes 39:126–131. doi:10.1007/s11262-009-0362-1

    Article  CAS  PubMed  Google Scholar 

  12. Klootwijk J, Klein I, Zabel P, van Kammen A (1977) Cowpea mosaic virus RNAs have neither m7G pppN … nor mono-, di- or triphosphates at their 5’ ends. Cell 11:73–82

    Article  CAS  PubMed  Google Scholar 

  13. Dereeper A, Guignon V, Blanc G et al (2008) Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res 36:W465–W469. doi:10.1093/nar/gkn180

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  14. Guindon S, Dufayard J-F, Lefort V et al (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59:307–321. doi:10.1093/sysbio/syq010

    Article  CAS  PubMed  Google Scholar 

  15. Zhang T, Jiang Y, Dong W (2014) A novel monopartite dsRNA virus isolated from the phytopathogenic fungus Ustilaginoidea virens and ancestrally related to a mitochondria-associated dsRNA in the green alga Bryopsis. Virology 462–463:227–235. doi:10.1016/j.virol.2014.06.003

    Article  PubMed  Google Scholar 

  16. Kotta-Loizou I, Sipkova J, Coutts RHA (2015) Identification and sequence determination of a novel double-stranded RNA mycovirus from the entomopathogenic fungus Beauveria bassiana. Arch Virol 160:873–875. doi:10.1007/s00705-014-2332-8

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The authors would like to thank the anonymous reviewers for their valuable comments and suggestions. This work was supported by Postdok_BIOGLOBE (CZ.1.07/2.3.00/30.0032), co-financed by the European Social Fund and the State Budget of the Czech Republic (to I.K and K.P) and project LH-13136 Kontakt II - “Mycovirus-host interactions in diseased isolates of phyto- and entomopathogenic micromycetes” from the Ministry of Education, Youth and Sports of the Czech Republic (to K.P).

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Authors and Affiliations

  1. Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, v.v.i., Branišovská 31, 370 05, České Budějovice, Czech Republic

    Igor Koloniuk, Lenka Hrabáková & Karel Petrzik

  2. Department of Genetics, Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 31a, 370 05, České Budějovice, Czech Republic

    Lenka Hrabáková

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  1. Igor Koloniuk
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  2. Lenka Hrabáková
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Correspondence to Igor Koloniuk.

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Koloniuk, I., Hrabáková, L. & Petrzik, K. Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana . Arch Virol 160, 1585–1588 (2015). https://doi.org/10.1007/s00705-015-2416-0

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  • DOI: https://doi.org/10.1007/s00705-015-2416-0

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