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A double-stranded RNA as the genome of a potential virus infecting Vicia faba

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Abstract

Preparations of double-stranded (ds) RNAs extracted from naturally infected Vicia faba Linn. growing in Hangzhou, Zhejiang Province, Eastern China displayed 3 dominant bands (FaR1, FaR2, and FaR3). FaR2 and FaR3 were found to be identical to the genomic dsRNAs of a recently reported Vicia cryptic virus (VCV). The positive strand of FaR1 contained two large open reading frames (ORFs), ORF1 and ORF2. The putative proteins encoded by these ORFs were found to have certain similarities to the putative capsid protein [ABO36237] and RNA-dependent RNA polymerase [ABC96788], respectively, of Tomato yellow stunt virus. Thus, FaR1 may represent the genome of a new dsRNA virus, which we have named Vicia cryptic virus M.

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References

  1. O.W. Barnett, Phytopathology 83, 33–34 (1993)

    CAS  Google Scholar 

  2. A.T. Jones, in Vicia faba Feeding Value, Processing and Viruses, ed. by D.A. Bond (M. Nijhoff, Den Haag, 1980), pp. 319–333

  3. R.H. Kenten, A.J. Cockbain, R.D. Woods, Rothamsted Exp. Stn. Rep. Part I, 222 (1978)

  4. M.A. Abou-Elnasr, A.T. Jones, M.A. Mayo, J. Gen. Virol. 66, 2453–2460 (1985). doi:https://doi.org/10.1099/0022-1317-66-11-2453

    Article  CAS  Google Scholar 

  5. R. Blawid, D. Stephan, E. Maiss, Arch. Virol. 152, 1477–1488 (2007). doi:https://doi.org/10.1007/s00705-007-0966-5

    Article  CAS  Google Scholar 

  6. P.R. Lambden, S.J. Cooke, E.O. Caul, I.N. Clarke, J. Virol. 66, 1817–1822 (1992)

    CAS  PubMed  PubMed Central  Google Scholar 

  7. H. Attoui, F. Billoir, J.F. Cantaloube, P. Biagini, P.D. de Micco, X. Lamballerie, J. Virol. Methods 89, 147–158 (2000). doi:https://doi.org/10.1016/S0166-0934(00)00212-3

    Article  CAS  Google Scholar 

  8. L. Chen, J.S. Chen, L. Liu, X. Yu, S. Yu, T.Z. Fu, W.H. Liu, Arch. Virol. 151, 849–859 (2006). doi:https://doi.org/10.1007/s00705-005-0685-8

    Article  CAS  Google Scholar 

  9. J. Sambrook, D.W. Russell, Molecular Cloning: A Laboratory Manual, 3rd edn. (CSHL Press, New York, 2001)

    Google Scholar 

  10. S.F. Altschul, T.L. Madden, A. Schaffer, A. Zhang, J.Z. Zhang, W. Miller, D.J. Lipman, Nucleic Acids Res. 25, 3389–3402 (1997). doi:https://doi.org/10.1093/nar/25.17.3389

    Article  CAS  Google Scholar 

  11. J.D. Tompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, D.G. Higgins, Nucleic Acids Res. 24, 4822–4876 (1997). doi:https://doi.org/10.1093/nar/25.24.4876

    Google Scholar 

  12. J.W. Kim, S.Y. Kim, K.M. Kim, Virus Genes 27, 249–256 (2003). doi:https://doi.org/10.1023/A:1026343831909

    Article  CAS  Google Scholar 

  13. M. Romoa, A. Leuchtmann, B. García, I. Zabalgogeazcoa, Virus Res. 124, 38–43 (2007). doi:https://doi.org/10.1016/j.virusres.2006.09.008

    Article  Google Scholar 

  14. N.M. Salem, D.A. Golino, B.W. Falk, A. Rowhani, Arch. Virol. 153, 455–462 (2008). doi:https://doi.org/10.1007/s00705-007-0008-3

    Article  CAS  Google Scholar 

  15. S. Sabanadzovic, R.A. Valverde, J.K. Brown, R.R. Martin, I.E. Tzanetakis, Virus Res. 140, 130–137 (2009). doi:https://doi.org/10.1016/j.virusres.2008.11.018

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (30671361). We thank Mr. Feng Xue for his assistance of the phylogenetic and sequence analysis; Dr. Zhiyou Du for his direction in the methods utilization; and Dr. Greg Duns for his critical reading of our manuscript.

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Authors and Affiliations

  1. Institute of Bioengineering, Zhejiang Sci-Tech University, Road No.2, Xiasha, Hangzhou, Zhejiang, 310018, People’s Republic of China

    Weixia Liu & Jishuang Chen

  2. Institute of Bioresource Engineering, Nanjing University of Technology, NO.5 Xinmofan Road, Nanjing, Jiangsu, 210009, People’s Republic of China

    Jishuang Chen

Authors
  1. Weixia Liu
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  2. Jishuang Chen
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Correspondence to Jishuang Chen.

Additional information

The GenBank Accession numbers of the sequences reported in this paper are EU605883, EU605884, and EU371896.

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Liu, W., Chen, J. A double-stranded RNA as the genome of a potential virus infecting Vicia faba . Virus Genes 39, 126–131 (2009). https://doi.org/10.1007/s11262-009-0362-1

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  • DOI: https://doi.org/10.1007/s11262-009-0362-1

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