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The complete genome sequence of a novel chrysovirus from the entomopathogenic fungus Beauveria bassiana Vuillemin

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Abstract

Beauveria bassiana, an entomopathogenic fungus, is used for arthropod pest control worldwide. Here, we report the discovery and characterization of a novel double-stranded RNA (dsRNA) mycovirus, Beauveria bassiana chrysovirus 2 (BbCV-2), isolated from a Chinese B. bassiana strain. The genome sequence of the virus was determined by metagenomic sequencing, RT-PCR, and RACE cloning and was found to consist of four dsRNA segments that are 3441 bp, 2779 bp, 2925 bp, and 2688 bp long, respectively. Each dsRNA segment contains a single ORF. The ORF of dsRNA1 encodes a 1114-amino-acid (aa) protein (123.4 kDa) with a conserved RNA-dependent RNA polymerase (RdRp) motif, the sequence of which showed the highest identity of only 16.13% to that of Beauveria bassiana chrysovirus-1 (BbCV-1). The ORF of dsRNA2 encodes an 805-aa coat protein (CP) (84.7 kDa). The ORFs of dsRNAs 3 and 4 encodes proteins of undetermined function. The virus is a new member of the family Chrysoviridae from B. bassiana.

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References

  1. Greenfield M, Gómez-Jiménez MI, Ortiz V, Vega FE, Kramer M, Parsa S (2016) Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation. Biol Control 95:40–48. https://doi.org/10.1016/j.biocontrol.2016.01.002

    Article  PubMed  PubMed Central  Google Scholar 

  2. Zhang Z, Lu Y, Xu W, Sui L, Du Q, Wang Y, Zhao Y, Li Q (2020) Influence of genetic diversity of seventeen Beauveria bassiana isolates from different hosts on virulence by comparative genomics. BMC Genom. https://doi.org/10.1186/s12864-020-06791-9

    Article  Google Scholar 

  3. Fernandes EK, Rangel DE, Braga GU, Roberts DW (2015) Tolerance of entomopathogenic fungi to ultraviolet radiation: a review on screening of strains and their formulation. Curr Genet 61:427–440. https://doi.org/10.1007/s00294-015-0492-z

    Article  CAS  PubMed  Google Scholar 

  4. Ugine TA (2011) The effect of temperature and exposure to Beauveria bassiana on tarnished plant bug Lygus lineolaris (Heteroptera: Miridae) population dynamics, and the broader implications of treating insects with entomopathogenic fungi over a range of temperatures. Biol Control 59:373–383. https://doi.org/10.1016/j.biocontrol.2011.08.004

    Article  Google Scholar 

  5. Kangassalo K, Valtonen TM, Roff D, Pölkki M, Dubovskiy IM, Sorvari J, Rantala MJ (2015) Intra- and trans-generational effects of larval diet on susceptibility to an entomopathogenic fungus, Beauveria bassiana, in the greater wax moth, Galleria mellonella. J Evol Biol 28:1453–1464. https://doi.org/10.1111/jeb.12666

    Article  CAS  PubMed  Google Scholar 

  6. Kotta-Loizou I, Coutts RHA (2017) Studies on the Virome of the entomopathogenic fungus Beauveria bassiana reveal novel dsRNA elements and mild hypervirulence. PLoS Pathog 13:e1006183. https://doi.org/10.1371/journal.ppat.1006183

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Kotta-Loizou I, Castón JR, Coutts RHA, Hillman BI, Jiang D, Kim DH, Moriyama H, Suzuki N (2020) ICTV virus taxonomy profile: Chrysoviridae. J Gen Virol 101:143–144. https://doi.org/10.1099/jgv.0.001383

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Shi N, Yang G, Wang P, Wang Y, Yu D, Huang B (2019) Complete genome sequence of a novel partitivirus from the entomogenous fungus Beauveria bassiana in China. Adv Virol 164:3141–3144. https://doi.org/10.1007/s00705-019-04428-1

    Article  CAS  Google Scholar 

  9. Kotta-Loizou I, Sipkova J, Coutts RHA (2015) Identification and sequence determination of a novel double-stranded RNA mycovirus from the entomopathogenic fungus Beauveria bassiana. Adv Virol 160:873–875. https://doi.org/10.1007/s00705-014-2332-8

    Article  CAS  Google Scholar 

  10. Koloniuk I, Hrabáková L, Petrzik K (2015) Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana. Adv Virol 160:1585–1588. https://doi.org/10.1007/s00705-015-2416-0

    Article  CAS  Google Scholar 

  11. Filippou C, Garrido-Jurado I, Meyling N, Quesada-Moraga E, Coutts RHA, Kotta-Loizou I (2018) Mycoviral population dynamics in Spanish isolates of the entomopathogenic fungus Beauveria bassiana. Viruses. https://doi.org/10.3390/v10120665

    Article  PubMed  PubMed Central  Google Scholar 

  12. Kimaru SK, Monda E, Cheruiyot RC, Mbaka J, Alakonya A (2018) Morphological and molecular identification of the causal agent of anthracnose disease of avocado in Kenya. Int J Microbiol 2018:1–10. https://doi.org/10.1155/2018/4568520

    Article  CAS  Google Scholar 

  13. Bar-Joseph M, Rosner A, Moscovitz M, Hull R (1983) A simple procedure for the extraction of double-stranded RNA from virus-infected plants. J Virol Methods 6:1–8. https://doi.org/10.1016/0166-0934(83)90062-9

    Article  CAS  PubMed  Google Scholar 

  14. Lambden PR, Cooke SJ, Caul EO, Clarke IN (1992) Cloning of noncultivatable human rotavirus by single primer amplification. J Virol 66:1817–1822. https://doi.org/10.1128/jvi.66.3.1817-1822.1992

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Marchler-Bauer A, Derbyshire MK, Gonzales NR, Lu S, Chitsaz F, Geer LY, Geer RC, He J, Gwadz M, Hurwitz DI, Lanczycki CJ, Lu F, Marchler GH, Song JS, Thanki N, Wang Z, Yamashita RA, Zhang D, Zheng C, Bryant SH (2015) CDD: NCBI’s conserved domain database. Nucleic Acids Res 43:D222-226. https://doi.org/10.1093/nar/gku1221

    Article  CAS  Google Scholar 

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Funding

This study was supported by Jilin Agricultural Science and Technology Innovation Project (Grant No.: CXGC 2017JQ016) and National Key R&D Programs (Grant No.: 2017YFD200608).

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Author notes

  1. Le Li and Qin Kang contributed equally to this work.

Authors and Affiliations

  1. Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture, Jilin Academy of Agricultural Sciences, Changchun, 130033, Jilin, People’s Republic of China

    Le Li, Qin Kang, Yang Lu, Li Sui, Zhengkun Zhang & Qiyun Li

  2. College of Plant Protection, Jilin Agricultural University, Changchun, 130118, People’s Republic of China

    Le Li & Qiyun Li

  3. Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, 100193, People’s Republic of China

    Qin Kang & Qiyun Li

  4. Hubei Engineering Research Center for Pest Forewarning and Management, Yangtze University, Jingzhou, 434025, Hubei, People’s Republic of China

    Songbai Zhang

  5. State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Du Hai

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  1. Le Li
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  2. Qin Kang
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  3. Songbai Zhang
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  4. Du Hai
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  6. Li Sui
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  7. Zhengkun Zhang
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Correspondence to Zhengkun Zhang or Qiyun Li.

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Handling Editor: Ioly Kotta-Loizou.

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Li, L., Kang, Q., Zhang, S. et al. The complete genome sequence of a novel chrysovirus from the entomopathogenic fungus Beauveria bassiana Vuillemin. Arch Virol 166, 3443–3447 (2021). https://doi.org/10.1007/s00705-021-05215-7

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  • DOI: https://doi.org/10.1007/s00705-021-05215-7

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