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Genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus Tospovirus

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Abstract

Chrysanthemum stem necrosis virus (CSNV) is a member of a tentative tospovirus species. In this study, the complete genomic sequence of the Japanese CSNV isolate TcCh07A was determined. The L RNA is 8960 nt long and encodes the 331.0-kDa RNA-dependent RNA polymerase. The M RNA is 4828 nt long and encodes the 34.1-kDa movement protein (NSm) and the 127.7-kDa glycoprotein precursor (Gn/Gc). The S RNA is 2949 nt long and encodes the 52.4-kDa silencing suppressor protein (NSs) and the 29.3-kDa nucleocapsid (N) protein. The N protein of CSNV-TcCh07A was purified from virus-infected plant tissues and used for production of a rabbit polyclonal antiserum (RAs) and a monoclonal antibody (MAb). Results of serological tests by indirect ELISA and western blotting using the prepared RAs and MAb and a previously produced RAs against the N protein of tomato spotted wilt virus (TSWV) indicated that CSNV-TcCh07A, TSWV, tomato chlorotic spot virus, groundnut ringspot virus, alstroemeria necrotic streak virus and impatiens necrotic spot virus are serologically related.

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Acknowledgments

We thank the virus providers: Dr. Dennis Gonsalves for GRSV; Dr. James W. Moyer for INSV; Dr. Richard Kormelink for ANSV, IYSV and TYRV; Dr. Prem A. Rajagopalan for GBNV and WBNV; Dr. Ioannis E. Tzanetakis for SVNaV; the DSMZ Plant Virus Collection, Germany, for TCSV; and the Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture, Executive Yuan, for permission to import the virus. We also thank Dr. Lei Wan for providing myeloma cells. Funding was provided by the National Science Council (NSC92-2317-B-005-024, NSC-101-2911-I-005-301 and NSC-102-2911-I-005-301) and the Ministry of Education, Taiwan, R.O.C. under the ATU plan for study.

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Authors and Affiliations

  1. Department of Biotechnology, Asia University, Wufeng, Taichung, 41354, Taiwan

    Pei-Ru Wu, Wan-Chu Chien, Yun-Chi Wang & Tsung-Chi Chen

  2. Agricultural Research Center, National Agriculture and Food Research Organization, Kannondai, Tsukuba, Ibaraki, 305-8666, Japan

    Mitsuru Okuda

  3. Laboratory of Plant Pathology, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka, 812-8581, Japan

    Minoru Takeshita

  4. Department of Plant Pathology, National Chung Hsing University, Taichung, 40227, Taiwan

    Shyi-Dong Yeh

  5. Agricultural Biotechnology Center, National Chung Hsing University, Taichung, 40227, Taiwan

    Shyi-Dong Yeh

  6. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, 40402, Taiwan

    Tsung-Chi Chen

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  1. Pei-Ru Wu
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  2. Wan-Chu Chien
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  3. Mitsuru Okuda
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  4. Minoru Takeshita
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Correspondence to Tsung-Chi Chen.

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Wu, PR., Chien, WC., Okuda, M. et al. Genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus Tospovirus . Arch Virol 160, 529–536 (2015). https://doi.org/10.1007/s00705-014-2287-9

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  • DOI: https://doi.org/10.1007/s00705-014-2287-9

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