Abstract
Three isolates of Chrysanthemum stem necrosis virus (CSNV) were obtained from chrysanthemum plants in distinct regions of Japan in 2006 and 2007. All the original host plants showed severe necrotic symptoms on the leaves and stems. Amino acid sequence data of the nucleocapsid protein genes of the three isolates (CbCh07A, TcCh07A, and GnCh07S) showed high identities with those of two other CSNV isolates, HiCh06A L1 from Japan and Chry1 from Brazil. Furthermore, for the first time the complete nucleotide sequence of the S RNA was determined for CSNV (isolate HiCh06A). In phylogenetic analysis based on the non-structural protein genes from the genus Tospovirus, HiCh06A L1 was placed in the same genetic group as Tomato spotted wilt virus (TSWV) and Impatiens necrotic spot virus. Host range examination for isolates HiCh06A L1 and CbCh07A showed that green pepper (cv. ‘Kyoyutaka’, ‘Saitamawase’, ‘Tosakatsura’, ‘L3 sarara’ and ‘L3 miogi’) and tomato (cv. ‘Sekaiichitomato’) were systemically susceptible hosts, whereas TSWV-resistant Solanaceae species, Capsicum chinense, Lycopersicon peruvianum and a TSWV-resistant cultivar of green pepper (cv. TSR miogi), were resistant.
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Acknowledgement
Authors would like to thank Mr. Shinichiro Okazaki, Oita Prefectural Agriculture, Forestry and Fisheries Research Center, for providing F. occidentalis and for the helpful suggestions with regard to the transmission tests, and Mr. Osamu Nunomura, Japan Horticultural Production and Research Institute, Division of extension for production, for providing ‘TSR miogi’. Germplasm of Capsicum chinense and Lycopersicon peruvianum was provided by National Institute of Agrobiological Sciences, Japan.
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Takeshita, M., Nagai, N., Okuda, M. et al. Molecular and biological characterization of Chrysanthemum stem necrosis virus isolates from distinct regions in Japan. Eur J Plant Pathol 131, 9–14 (2011). https://doi.org/10.1007/s10658-011-9797-z
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DOI: https://doi.org/10.1007/s10658-011-9797-z