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Identification and characterization of Lisianthus necrotic ringspot virus, a novel distinct tospovirus species causing necrotic disease of lisianthus (Eustoma grandiflorum)

  • Viral and Viroid Diseases
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Abstract

In 2007, lisianthus (Eustoma grandiflorum) plants with necrotic ringspots on the leaves were found in Kochi Prefecture, Japan. Tospovirus-like spherical enveloped particles with ca. 160 nm in diameter were observed with electron microscopy. The complete nucleotide sequence of the S RNA segment of the virus was determined, and phylogenetic analysis using deduced amino acid sequences of the nucleocapsid protein and the nonstructural S protein indicated that the virus is phylogenetically distinct from any known tospovirus species. The results suggest that the virus is a new member of the genus Tospovirus, in the family Bunyaviridae. The virus is the fifth distinct tospovirus occurring naturally in lisianthus in Japan. The necrotic symptoms were reproduced on lisianthus seedlings after mechanical inoculation. The host range of the virus isolate on several test plants was also examined.

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Acknowledgments

The authors thank Dr. Richard Kormelink, Laboratory of Virology, Wageningen University for valuable suggestions to our study.

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Authors and Affiliations

  1. Kochi Agricultural Research Center, 1100 Hataeda, Nankoku, Kochi, 783-0023, Japan

    Yoshifumi Shimomoto

  2. Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan

    Kappei Kobayashi

  3. National Agriculture and Food Research Organization, Kannondai, Tsukuba, Ibaraki, 305-8666, Japan

    Mitsuru Okuda

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  1. Yoshifumi Shimomoto
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  2. Kappei Kobayashi
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  3. Mitsuru Okuda
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Correspondence to Yoshifumi Shimomoto.

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Shimomoto, Y., Kobayashi, K. & Okuda, M. Identification and characterization of Lisianthus necrotic ringspot virus, a novel distinct tospovirus species causing necrotic disease of lisianthus (Eustoma grandiflorum). J Gen Plant Pathol 80, 169–175 (2014). https://doi.org/10.1007/s10327-014-0503-9

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  • DOI: https://doi.org/10.1007/s10327-014-0503-9

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