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The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28

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Abstract

The complete nucleotide sequence of Alternaria longipes dsRNA virus 1 (AlRV1), a novel double-stranded RNA (dsRNA) mycovirus, was determined and analyzed. AlRV1-HN28 contains a single dsRNA genome segment 3415 base pairs in length (excluding the 3′ poly(A) tail) and was predicted to contain two discontiguous open reading frames (ORFs, ORF A and ORF B). The 5′-proximal ORF A (1182 nt) potentially encodes a protein of 394 amino acids (aa) with a predicted molecular mass of 43 kDa; this protein showed no significant similarities to any other sequences in any of the NCBI protein databases. The 3′-proximal ORF B (1737 nt) encodes a protein of 579 aa with a predicted molecular mass of 65 kDa; this protein sequence shares similarities with the conserved domains of RNA-dependent RNA polymerases of other mycoviruses. Phylogenetic analysis indicated that AlRV1-HN28 was closely related to four other unclassified viruses, which suggests that the AlRV1-HN28 isolated from Alternaria longipes may belong to a new family of dsRNA mycoviruses. This is the first report of the full-length nucleotide sequence of a mycovirus that infects Alternaria longipes.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31171818). We greatly thank Dr. Meng Zhang at Henan Agricultural University, Zhengzhou, Henan, China, for assistance with fungal identification.

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Authors and Affiliations

  1. Key Laboratory at Universities of Xinjiang, Uygur Autonomous Region for Oasis, Agricultural Pest Management and Plant Protection Resource Utilization, Shihezi University, Shihezi, 832003, China

    Yanhong Lin & Shengxue Liu

  2. The Institute of Plant Protection/State Key Laboratory for Biology of Plant Diseases and Insect Pests, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Hailong Zhang, Chengjin Zhao & Lihua Guo

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  1. Yanhong Lin
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  2. Hailong Zhang
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  3. Chengjin Zhao
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  4. Shengxue Liu
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  5. Lihua Guo
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Correspondence to Shengxue Liu or Lihua Guo.

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Lin, Y., Zhang, H., Zhao, C. et al. The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28. Arch Virol 160, 577–580 (2015). https://doi.org/10.1007/s00705-014-2218-9

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  • DOI: https://doi.org/10.1007/s00705-014-2218-9

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