Abstract
The prevalence of porcine sapoviruses (SaVs) and noroviruses (NoVs) in nursing piglets on three pig farms in Ohio was studied. Fecal samples (n = 139) were collected from individual pigs and screened for caliciviruses by RT-PCR. Phylogenetic analysis was conducted using partial sequences of the RNA polymerase region. Three different SaV genogroups, including a newly emerging one (DO19 Korea-like) were detected. No NoVs were detected. Kobuviruses, emerging members of the family Picornaviridae, were detected by primers designed for SaV. To our knowledge, this is the first report of porcine DO19 Korea-like SaV and kobuvirus in the United States.
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Acknowledgments
This work was supported in part by a National Institutes of Health-Fogarty Grant (project GRT00117993/60023878). Salaries and research support were provided by state and federal funds provided to the Ohio Agricultural Research and Development Center (OARDC), The Ohio State University. Special thanks to Kelly Scheuer, for technical assistance and assistance in manuscript preparation, and Anastasia Vlasova and Kwonil Jung for their critical review of this manuscript. We also thank Joshua Amimo for help in sample preparation.
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Sisay, Z., Wang, Q., Oka, T. et al. Prevalence and molecular characterization of porcine enteric caliciviruses and first detection of porcine kobuviruses in US swine. Arch Virol 158, 1583–1588 (2013). https://doi.org/10.1007/s00705-013-1619-5
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DOI: https://doi.org/10.1007/s00705-013-1619-5