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Molecular detection of porcine kobuviruses in pigs in Korea and their association with diarrhea

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Abstract

Kobuviruses are small, non-enveloped viruses with a single-stranded, positive-sense genomic RNA, belonging to the family Picornaviridae, a highly diverse family of important pathogens of human and other animals. Porcine kobuvirus has been found recently, and consequently, information about the virus is lacking. In this study, we identified porcine kobuviruses from pigs in Korea by RT-PCR, cloning and sequencing, and we showed the existence of genetic diversity among geographically separated porcine kobuviruses through genetic and phylogenetic analysis. Epidemiological studies of porcine kobuvirus linked to diarrhea indicated that porcine kobuvirus infections are endemic in diarrheic pigs in Korea. Statistical analysis of the porcine kobuvirus positive rate between diarrheic and healthy pigs as well as a survey for other enteric pathogens in diarrheic pigs suggests that porcine kobuvirus may play a role as a causative agent of gastroenteritis in pigs.

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Acknowledgments

This work was supported by a grant (Code #20070401034009) from BioGreen 21 Program, Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Veterinary Medicine Virology Lab, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, 151-742, Korea

    Seong-Jun Park, Hye-Kwon Kim, Se-Mi Rho, Jae-Yeon Han, Van-Giap Nguyen & Bong-Kyun Park

  2. Research Unit, Green Cross Veterinary Products, Yong-In, 449-903, Korea

    Hyoung-Joon Moon & Dae-Sub Song

Authors
  1. Seong-Jun Park
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  2. Hye-Kwon Kim
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  3. Hyoung-Joon Moon
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  4. Dae-Sub Song
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  5. Se-Mi Rho
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  6. Jae-Yeon Han
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  7. Van-Giap Nguyen
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  8. Bong-Kyun Park
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Corresponding author

Correspondence to Bong-Kyun Park.

Additional information

Nucleotide sequence data reported is available in the GenBank database under the accession nos. GU723955 and GU723956.

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Park, SJ., Kim, HK., Moon, HJ. et al. Molecular detection of porcine kobuviruses in pigs in Korea and their association with diarrhea. Arch Virol 155, 1803–1811 (2010). https://doi.org/10.1007/s00705-010-0774-1

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  • DOI: https://doi.org/10.1007/s00705-010-0774-1

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