Skip to main content
SpringerLink
Log in

Molecular insights into the phylogeny of canine parvovirus 2 (CPV-2) with emphasis on Korean isolates: a Bayesian approach

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

A Bayesian approach was utilized to further investigate the molecular phylogeny of canine parvovirus 2 (CPV-2) with emphasis on Korean isolates, and the complete VP2 gene sequences for 23 Korean isolates in 2007 and two vaccine strains were determined. Of the 44 Korean CPVs, 40 were identified as CPV-2a, three as CPV-2b, and the remaining one as a CPV-2a variant having a Gly300Asp mutation. Compared with CPV-2a, our findings also showed the presence of six additional amino acid substitutions (Thr440Ala, Ile418Thr, Pro435Ser, Asp413Asn, Thr322Ser, and Tyr324Ile) within the VP2 gene of the Korean isolates. In the phylogenetic tree, the overall Korean CPVs did not emerge as an independent clade within the global CPVs, nor did they show a close relationship to the CPVs from any other country. Our VP2 sequence data also revealed no geographic influence on the spread of CPVs worldwide. dN/dS analysis showed that purifying selection is acting on the VP2 gene of Korean CPVs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Abascal F, Zardoya R, Posada D (2005) Pro test: selection of best-fit models of protein evolution. Bioinformatics 20:2104–2105

    Article  Google Scholar 

  2. Akaike H (1974) A new look at the statistical model identification. IEEE Trans Autom Control 19:716–723

    Article  Google Scholar 

  3. Appel MJG, Scott FW, Carmichael LE (1979) Isolation and immunization studies of a canine parvo-like virus from dogs with haemorrhagic enteritis. Vet Rec 105:156–159

    PubMed  CAS  Google Scholar 

  4. Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia D, Bozzo G, Elia G, Decaro N, Carmichael L (2001) Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol 82:3021–3025

    PubMed  CAS  Google Scholar 

  5. Chapman MS, Rossmann MG (1993) Structure, sequence, and function correlations among parvoviruses. Virology 194:491–508

    Article  PubMed  CAS  Google Scholar 

  6. Decaro N, Martella V, Desario C, Bellacicco AL, Camero M, Manna L, d’Aloja D, Buonavoglia C (2006) First detection of canine parvovirus type 2c in pups with haemorrhagic enteritis in Spain. J Vet Med 53:468–472

    Article  CAS  Google Scholar 

  7. Hall TA (1999) BIOEDIT: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  8. Huelsenbeck JP, Ronquist F (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    Article  PubMed  CAS  Google Scholar 

  9. Ikeda Y, Mochizuki M, Naito R, Nakamura K, Miyazawa T, Mikami T, Takahashi E (2000) Predominance of canine parvovirus (CPV) in unvaccinated cat populations and emergence of new antigenic types of CPVs in cats. Virology 5:13–19

    Article  Google Scholar 

  10. Kang BK, Song DS, Lee CS, Jung KI, Park SJ, Kim EM, Park BK (2008) Prevalence and genetic characterization of canine parvoviruses in Korea. Virus Genes 36:127–133

    Article  PubMed  CAS  Google Scholar 

  11. Kapil S, Cooper E, Lamm C, Murray B, Rezabek G, Johnston L 3rd, Campbell G, Johnson B (2007) Canine parvovirus types 2c and 2b circulating in North American dogs in 2006 and 2007. J Clin Microbiol 45:4044–4047

    Article  PubMed  CAS  Google Scholar 

  12. Ku JL, Seo IB, Lim CH (1994) Histopathological and immunohistochemical studies on the intestinal lesions related to the infected age in spontaneous canine parvovirus enteritis. Korean J Vet Res 34:537–547

    Google Scholar 

  13. Martella V, Decaro N, Buonavoglia C (2006) Evolution of CPV-2 and implication for antigenic/genetic characterization. Virus Genes 33:11–13

    Article  PubMed  CAS  Google Scholar 

  14. Mochizuki M, Horiuchi M, Hiragi H, San Gabriel MC, Yasuda N, Uno T (1996) Isolation of canine parvovirus from a cat manifesting clinical signs of feline panleukopenia. J Clin Microbiol 34:2101–2105

    PubMed  CAS  Google Scholar 

  15. Moon HS, Lee SA, Lee SG, Choi R, Jeoung SY, Kim D, Hyun C (2008) Comparison of the pathogenicity in three different Korean canine parvovirus 2 (CPV-2) isolates. Vet Microbiol 131:47–56

    Article  PubMed  CAS  Google Scholar 

  16. Nakamura M, Tohya Y, Miyazawa T, Mochizuki M, Phung HT, Nguyen NH, Huynh LM, Nguyen LT, Nguyen PN, Nguyen PV, Nguyen NP, Akashi H (2004) A novel antigenic variant of Canine parvovirus from a Vietnamese dog. Arch Virol 149:2261–2269

    Article  PubMed  CAS  Google Scholar 

  17. Nielsen R, Yang Z (1998) Likelihood models for detecting positively selected amino acid sites and applications to the HIV-1 envelope gene. Genetics 148:929–936

    PubMed  CAS  Google Scholar 

  18. Page RDM (1996) TREEVIEW: an application to display phylogenetic trees on a personal computer. Comput Appl Biosci 12:357–358

    PubMed  CAS  Google Scholar 

  19. Park JH, Song JY, Lee JB, Hyun BH, An SH, Jun MH (1992) Restriction endonuclease analysis of canine parvovirus DNA isolated in Korea. Korean J Vet Res 32:597–603

    Google Scholar 

  20. Parrish CR (1991) Mapping specific functions in the capsid structure of canine parvovirus and feline panleukopenia virus using infectious plasmid clones. Virology 183:195–205

    Article  PubMed  CAS  Google Scholar 

  21. Parrish CR, Aquadro CF, Strassheim ML, Evermann JF, Sgro JY, Mohammed HO (1991) Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus. J Virol 65:6544–6552

    PubMed  CAS  Google Scholar 

  22. Pereira CA, Leal ES, Durigon EL (2007) Selective regimen shift and demographic growth increase associated with the emergence of high-fitness variants of canine parvovirus. Infect Genet Evol 7:399–409

    Article  PubMed  CAS  Google Scholar 

  23. Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818

    Article  PubMed  CAS  Google Scholar 

  24. Qin Q, Loeffler IK, Li M, Tian K, Wei F (2007) Sequence analysis of a canine parvovirus isolated from a red panda (Ailurus fulgens) in China. Virus Genes 34:299–302

    Article  PubMed  CAS  Google Scholar 

  25. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574

    Article  PubMed  CAS  Google Scholar 

  26. Shackelton LA, Parrish CR, Truyen U, Holmes EC (2005) High rate of viral evolution associated with the emergence of carnivore parvovirus. Proc Natl Acad Sci 102:379–384

    Article  PubMed  CAS  Google Scholar 

  27. Strimmer K, von Haeseler A (1996) Quartet puzzling: a quartet maximum likelihood method for reconstructing tree topologies. Mol Biol Evol 13:964–969

    CAS  Google Scholar 

  28. Suyama M, Torrents D, Bork P (2006) PAL2NAL: robust conversion of protein sequence alignments into the corresponding codon alignments. Nucleic Acids Res: W609–W612. Nucleic Acids Res 34:W609–W612

    Article  PubMed  CAS  Google Scholar 

  29. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal-windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 22:4673–4680

    Article  Google Scholar 

  30. Wang HC, Chen WD, Lin SL, Chan JP, Wong ML (2005) Phylogenetic analysis of canine parvovirus VP2 gene in Taiwan. Virus Genes 31:171–174

    Article  PubMed  CAS  Google Scholar 

  31. Yang Z (1997) PAML: a program package for phylogenetic analysis by maximum likelihood. Comput Appl Biosci 13:555–556

    PubMed  CAS  Google Scholar 

  32. Yuan W, Parrish CR (2000) Comparison of two single-chain antibodies that neutralize canine parvovirus: analysis of an antibody-combining site and mechanisms of neutralization. Virology 269:471–480

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr. B.-K. Kang (Green Cross Veterinary Products, Korea) for providing the VP2 gene sequences that appeared in his paper (Kang et al. 2008). This project was funded by the National Veterinary Research and Ouarantine Service (NVRQS), Ministry for Food, Agriculture, Forestry and Fisheries (MFAFF), Republic of Korea.

Author information

Authors and Affiliations

  1. Division of Veterinary Biotechnology, National Veterinary Research and Quarantine Service, 480, Anyang 6-dong, Anyang, Gyeonggido, 430-824, South Korea

    Sook Hee Yoon, Wooseog Jeong, Hyun-Jeong Kim & Dong-Jun An

Authors
  1. Sook Hee Yoon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wooseog Jeong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hyun-Jeong Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dong-Jun An
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dong-Jun An.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yoon, S.H., Jeong, W., Kim, HJ. et al. Molecular insights into the phylogeny of canine parvovirus 2 (CPV-2) with emphasis on Korean isolates: a Bayesian approach. Arch Virol 154, 1353–1360 (2009). https://doi.org/10.1007/s00705-009-0444-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-009-0444-3

Keywords

Search

Navigation