Abstract
Repeated stress engenders behavioral sensitization. The mesolimbic dopamine system is critically involved in drug-induced behavioral sensitization. In the present study we examined the differences between adolescent and adult rats in stress-induced behavioral sensitization to amphetamine and changes in dopamine (DA) and its metabolite levels in the mesolimbic system. Adolescent or adult rats were restrained for 2 h, once a day, for 7 days. Three days after the last exposure to stress, the animals were challenged with saline or amphetamine (1.0 mg/kg i.p.) and amphetamine-induced locomotion was recorded for 40 min. Immediately after the behavioral tests, rats were decapitated and the nucleus accumbens (NAcc), ventral tegmental area (VTA) and amygdala (AM) were removed to measure tissue levels of DA and its metabolites by HPLC. Exposure to repeated restraint stress promoted behavioral sensitization to amphetamine in both adult and adolescent rats. In adult rats, amphetamine administration increased DA levels in both the stress and control groups in the NAcc and VTA. In adolescent rats, amphetamine increased DA levels in the NAcc in rats exposed to stress. Furthermore, in the AM of adolescent rats in the control group, amphetamine increased the DA levels; however, amphetamine reduced this neurotransmitter in the rats that were exposed to stress. No alteration was observed in the dopamine metabolite levels. Therefore, stress promoted behavioral sensitization to amphetamine and this may be related to changes in DA levels in the mesolimbic system. These changes appear to be dependent on ontogeny.
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References
Adriani W, Laviola G (2000) A unique hormonal and behavioral hyporesponsivity to both forced novelty and d-amphetamine in periadolescent mice. Neuropharmacology 39:334–346
Antelman SM, Eichler AJ, Black CA, Kocan D (1980) Interchangeability of stress and amphetamine in sensitization. Science 207:329–331
Araujo AP, DeLucia R, Scavone C, Planeta CS (2003) Repeated predictable or unpredictable stress: effects on cocaine-induced locomotion and cyclic AMP-dependent protein kinase activity. Behav Brain Res 139:75–81
Armando AP, Lemoine EI, Segura MB (1993) The stress-induced reduction in monoamine oxidase (MAO) A activity is reversed by benzodiazepines: role of peripheral benozodiazepine receptors. Cell Mol Neurobiol 13:593–601
Bolanos CA, Glatt SJ, Jackson D (1998) Subsensitivity to dopaminergic drugs in periadolescent rats: a behavioral and neurochemical analysis. Brain Res Dev Brain Res 111:25–33
Cadoni C, Solinas M, Valentini V, Di Chiara G (2003) Selective psychostimulant sensitization by food restriction: differential changes in accumbens shell and core dopamine. Eur J Neurosci 18:2326–2334
Colussi-Mas J, Geisler S, Zimmer L, Zahm DS, Berod A (2007) Activation of afferents to the ventral tegmental area in response to acute amphetamine: a double-labelling study. Eur J Neurosci 26:1011–1025
Covington HE 3rd, Miczek KA (2001) Repeated social-defeat stress, cocaine or morphine. Effects on behavioral sensitization and intravenous cocaine self-administration “binges”. Psychopharmacology (Berl) 158:388–398
Covington HE 3rd, Miczek KA (2005) Intense cocaine self-administration after episodic social defeat stress, but not after aggressive behavior: dissociation from corticosterone activation. Psychopharmacology (Berl) 183:331–340
Cruz FC, Marin MT, Planeta CS (2008) The reinstatement of amphetamine-induced place preference is long-lasting and related to decreased expression of AMPA receptors in the nucleus accumbens. Neuroscience 151:313–319
de Jong JG, Wasilewski M, van der Vegt BJ, Buwalda B, Koolhaas JM (2005) A single social defeat induces short-lasting behavioral sensitization to amphetamine. Physiol Behav 83:805–811
Der-Avakian A, Bland ST, Schmid MJ, Watkins LR, Spencer RL, Maier SF (2006) The role of glucocorticoids in the uncontrollable stress-induced potentiation of nucleus accumbens shell dopamine and conditioned place preference responses to morphine. Psychoneuroendocrinology 31:653–663
Deroche V, Piazza PV, Maccari S, Le Moal M, Simon H (1992) Repeated corticosterone administration sensitizes the locomotor response to amphetamine. Brain Res 584:309–313
Deroche V, Piazza PV, Le Moal M, Simon H (1994) Social isolation-induced enhancement of the psychomotor effects of morphine depends on corticosterone secretion. Brain Res 640:136–139
Dhingra NK, Raju TR, Meti BL (1997) Selective reduction of monoamine oxidase A and B in the frontal cortex of subordinate rats. Brain Res 758:237–240
Diaz-Otanez CS, Capriles NR, Cancela LM (1997) D1 and D2 dopamine and opiate receptors are involved in the restraint stress-induced sensitization to the psychostimulant effects of amphetamine. Pharmacol Biochem Behav 58:9–14
Elliott BM, Faraday MM, Phillips JM, Grunberg NE (2004) Effects of nicotine on elevated plus maze and locomotor activity in male and female adolescent and adult rats. Pharmacol Biochem Behav 77:21–28
Giorgi O, Piras G, Lecca D, Corda MG (2005a) Behavioural effects of acute and repeated cocaine treatments: a comparative study in sensitisation-prone RHA rats and their sensitisation-resistant RLA counterparts. Psychopharmacology (Berl) 180:530–538
Giorgi O, Piras G, Lecca D, Corda MG (2005b) Differential activation of dopamine release in the nucleus accumbens core and shell after acute or repeated amphetamine injections: a comparative study in the Roman high- and low-avoidance rat lines. Neuroscience 135:987–998
Haile CN, GrandPre T, Kosten TA (2001) Chronic unpredictable stress, but not chronic predictable stress, enhances the sensitivity to the behavioral effects of cocaine in rats. Psychopharmacology (Berl) 154:213–220
Herman JP, Stinus L, Le Moal M (1984) Repeated stress increases locomotor response to amphetamine. Psychopharmacology (Berl) 84:431–435
Huang B, Dawson DA, Stinson FS, Hasin DS, Ruan WJ, Saha TD, Smith SM, Goldstein RB, Grant BF (2006) Prevalence, correlates, and comorbidity of nonmedical prescription drug use and drug use disorders in the United States: results of the National Epidemiologic Survey on Alcohol and Related Conditions. J Clin Psychiatry 67:1062–1073
Imperato A, Puglisi-Allegra S, Casolini P, Zocchi A, Angelucci L (1989) Stress-induced enhancement of dopamine and acetylcholine release in limbic structures: role of corticosterone. Eur J Pharmacol 165:337–338
Izenwasser S, French D (2002) Tolerance and sensitization to the locomotor-activating effects of cocaine are mediated via independent mechanisms. Pharmacol Biochem Behav 73:877–882
Kabbaj M, Norton CS, Kollack-Walker S, Watson SJ, Robinson TE, Akil H (2001) Social defeat alters the acquisition of cocaine self-administration in rats: role of individual differences in cocaine-taking behavior. Psychopharmacology (Berl) 158:382–387
Kalivas PW, Duffy P, Barrow J (1989) Regulation of the mesocorticolimbic dopamine system by glutamic acid receptor subtypes. J Pharmacol Exp Ther 251:378–387
Kita T, Miyazaki I, Asanuma M, Takeshima M, Wagner GC (2009) Dopamine-induced behavioral changes and oxidative stress in methamphetamine-induced neurotoxicity. Int Rev Neurobiol 88:43–64
Koob GF (2009) Brain stress systems in the amygdala and addiction. Brain Res 1293:61–75
Koob G, Kreek MJ (2007) Stress, dysregulation of drug reward pathways, and the transition to drug dependence. Am J Psychiatry 164:1149–1159
Lanier LP, Isaacson RL (1977) Early developmental changes in the locomotor response to amphetamine and their relation to hippocampal function. Brain Res 126:567–575
Lepsch LB, Gonzalo LA, Magro FJ, Delucia R, Scavone C, Planeta CS (2005) Exposure to chronic stress increases the locomotor response to cocaine and the basal levels of corticosterone in adolescent rats. Addict Biol 10:251–256
Marin MT, Cruz FC, Planeta CS (2008) Cocaine-induced behavioral sensitization in adolescent rats endures until adulthood: lack of association with GluR1 and NR1 glutamate receptor subunits and tyrosine hydroxylase. Pharmacol Biochem Behav 91:109–114
Marinelli M, Piazza PV (2002) Interaction between glucocorticoid hormones, stress and psychostimulant drugs. Eur J Neurosci 16:387–394
Mathews IZ, McCormick CM (2007) Female and male rats in late adolescence differ from adults in amphetamine-induced locomotor activity, but not in conditioned place preference for amphetamine. Behav Pharmacol 18:641–650
Mathews IZ, Waters P, McCormick CM (2009) Changes in hyporesponsiveness to acute amphetamine and age differences in tyrosine hydroxylase immunoreactivity in the brain over adolescence in male and female rats. Dev Psychobiol 51:417–428
Mendez IA, Williams MT, Bhavsar A, Lu AP, Bizon JL, Setlow B (2009) Long-lasting sensitization of reward-directed behavior by amphetamine. Behav Brain Res 201:74–79
Miczek KA, Yap JJ, Covington HE 3rd (2008) Social stress, therapeutics and drug abuse: preclinical models of escalated and depressed intake. Pharmacol Ther 120:102–128
Napolitano A, Cesura AM, Da Prada M (1995) The role of monoamine oxidase and catechol O-methyltransferase in dopaminergic neurotransmission. J Neural Transm 45:35–45
Nelson AM, Larson GA, Zahniser NR (2009) Low or high cocaine responding rats differ in striatal extracellular dopamine levels and dopamine transporter number. J Pharmacol Exp Ther 331:985–997
Nikulina EM, Covington HE 3rd, Ganschow L, Hammer RP Jr, Miczek KA (2004) Long-term behavioral and neuronal cross-sensitization to amphetamine induced by repeated brief social defeat stress: Fos in the ventral tegmental area and amygdala. Neuroscience 123:857–865
Nikulina EM, Miczek KA, Hammer RP Jr (2005) Prolonged effects of repeated social defeat stress on mRNA expression and function of mu-opioid receptors in the ventral tegmental area of rats. Neuropsychopharmacology 30:1096–1103
Pacchioni AM, Gioino G, Assis A, Cancela LM (2002) A single exposure to restraint stress induces behavioral and neurochemical sensitization to stimulating effects of amphetamine: involvement of NMDA receptors. Ann NY Acad Sci 965:233–246
Pacchioni AM, Cador M, Bregonzio C, Cancela LM (2007) A glutamate–dopamine interaction in the persistent enhanced response to amphetamine in nucleus accumbens core but not shell following a single restraint stress. Neuropsychopharmacology 32:682–692
Paxinos G, Watson C (2006) The rat forebrain in stereotaxic coordinates, 5th edn. Academic Press, USA
Perugini M, Vezina P (1994) Amphetamine administered to the ventral tegmental area sensitizes rats to the locomotor effects of nucleus accumbens amphetamine. J Pharmacol Exp Ther 270:690–696
Pierce RC, Kalivas PW (1997) A circuitry model of the expression of behavioral sensitization to amphetamine-like psychostimulants. Brain Res Brain Res Rev 25:192–216
Prasad BM, Sorg BA, Ulibarri C, Kalivas PW (1995) Sensitization to stress and psychostimulants. Involvement of dopamine transmission versus the HPA axis. Ann NY Acad Sci 771:617–625
Robinson TE, Berridge KC (1993) The neural basis of drug craving: an incentive-sensitization theory of addiction. Brain Res Brain Res Rev 18:247–291
Santos GC, Marin MT, Cruz FC, Delucia R, Planeta CS (2009) Amphetamine- and nicotine-induced cross-sensitization in adolescent rats persists until adulthood. Addict Biol 14:270–275
Schramm-Sapyta NL, Walker QD, Caster JM, Levin ED, Kuhn CM (2009) Are adolescents more vulnerable to drug addiction than adults? Evidence from animal models. Psychopharmacology (Berl) 206:1–21
Shahbazi M, Moffett AM, Williams BF, Frantz KJ (2008) Age- and sex-dependent amphetamine self-administration in rats. Psychopharmacology (Berl) 196:71–81
Sinha R (2008) Chronic stress, drug use, and vulnerability to addiction. Ann NY Acad Sci 1141:105–130
Spear LP (2000) The adolescent brain and age-related behavioral manifestations. Neurosci Biobehav Rev 24:417–463
Tirelli E, Tambour S, Michel A (2003) Sensitised locomotion does not predict conditioned locomotion in cocaine-treated mice: further evidence against the excitatory conditioning model of context-dependent sensitisation. Eur Neuropsychopharmacol 13:289–296
Vidal J, Bie J, Granneman RA, Wallinga AE, Koolhaas JM, Buwalda B (2007) Social stress during adolescence in Wistar rats induces social anxiety in adulthood without affecting brain monoaminergic content and activity. Physiol Behav 92:824–830
Yap JJ, Miczek KA (2007) Social defeat stress, sensitization, and intravenous cocaine self-administration in mice. Psychopharmacology (Berl) 192:261–273
Acknowledgments
The authors appreciate the excellent technical assistance by Elisabete Z.P. Lepera, Francisco Rocateli and Rosana F.P. Silva. This work was supported by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP-2007/08087-7).
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Cruz, F.C., Marin, M.T., Leão, R.M. et al. Stress-induced cross-sensitization to amphetamine is related to changes in the dopaminergic system. J Neural Transm 119, 415–424 (2012). https://doi.org/10.1007/s00702-011-0720-8
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DOI: https://doi.org/10.1007/s00702-011-0720-8