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Comparative analysis of diversification and population structure of kenaf (Hibiscus cannabinus L.) and roselle (H. sabdariffa L.) using SSR and RGA (resistance gene analogue) markers

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Abstract

Multiple DNA marker systems and complementary analytical approaches are often useful in population genetic analysis and speciation of plants. We investigated population structure of kenaf (Hibiscus cannabinus) and roselle (H. sabdariffa) for gaining insight in evolution and geographic separation of these crop species using SSR and RGA (resistance gene analogues) markers through Bayesian clustering and principal coordinate analysis (PCoA) methods. Genotyping by 12 SSR and 16 RGA markers amplified a total of 172 loci in the study population. The RGA markers generated higher number of alleles per marker (8.2) as compared to SSR (3.4), but exhibited lower heterozygosity in the population. Genetic variance and heterozygosity in roselle population for both marker systems were lower than in kenaf. RGA markers revealed higher variation among populations. Bayesian structure as well as PCoA analysis using RGA marker revealed distinct cluster for roselle, while SSR-based classification revealed high admixture. Results indicate geographic isolation and natural selection for adaptive RGA alleles in kenaf. The Indian kenaf landraces were distinct from the exotic kenaf accessions, suggesting separate lineage formation by geographic separation. Possible origin and domestication of roselle in South India is proposed.

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Acknowledgments

We thank Director, Central Research Institute for Jute and Allied Fibres for kind support to the present research work and the reviewers for their constructive comments.

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  1. Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata, 700120, India

    Pratik Satya, Maya Karan, Kaustav Chakraborty, Chinmay Biswas & P. G. Karmakar

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  1. Pratik Satya
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  2. Maya Karan
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  3. Kaustav Chakraborty
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  4. Chinmay Biswas
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  5. P. G. Karmakar
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Correspondence to Pratik Satya.

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Satya, P., Karan, M., Chakraborty, K. et al. Comparative analysis of diversification and population structure of kenaf (Hibiscus cannabinus L.) and roselle (H. sabdariffa L.) using SSR and RGA (resistance gene analogue) markers. Plant Syst Evol 300, 1209–1218 (2014). https://doi.org/10.1007/s00606-013-0956-x

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