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Assessment of molecular diversity and evolutionary relationship of kenaf (Hibiscus cannabinus L.), roselle (H. sabdariffa L.) and their wild relatives

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Abstract

Kenaf (Hibiscus cannabinus L.) and roselle (H. sabdariffa L.) are valuable fibre crop species with diverse end use. Phylogenetic relationship of 73 accessions of kenaf, roselle and their wild relatives from 15 countries was assessed using 44 inter-simple sequence repeat (ISSR) and jute (Corchorus olitorius L.) specific simple sequence repeats (SSR) markers. A total of 113 alleles were identified of which 61.95 % were polymorphic. Jute specific SSR markers exhibited high polymorphism and resolving power in kenaf, although ISSR markers exhibited higher resolving power than SSR markers. Number of polymorphic alleles varied from 1 to 5 for ISSR and 1 to 6 for SSR markers. Cultivated species exhibited higher allele polymorphism (57 %) than the wild species (35 %), but the improved cultivars exhibited lower genetic diversity compared to germplasm accessions. Accessions with common genetic lineage and geographical distribution clustered together. Indian kenaf varieties were distinct from cultivars bred in other countries and shared more genetic homology with African accessions. High genetic diversity was observed in the Indian (J = 0.35–0.74) and exotic kenaf germplasm collections (J = 0.38–0.79), suggesting kenaf might have been introduced in India from Africa through Central Asia during early domestication. Genetic similarity-based cluster analysis was in close accordance with taxonomic classification of Hibiscus.

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Authors and Affiliations

  1. Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata, 700120, India

    Pratik Satya, M. Karan, C. S. Kar, A. K. Mahapatra & B. S. Mahapatra

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  1. Pratik Satya
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  2. M. Karan
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  3. C. S. Kar
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  4. A. K. Mahapatra
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  5. B. S. Mahapatra
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Correspondence to Pratik Satya.

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Satya, P., Karan, M., Kar, C.S. et al. Assessment of molecular diversity and evolutionary relationship of kenaf (Hibiscus cannabinus L.), roselle (H. sabdariffa L.) and their wild relatives. Plant Syst Evol 299, 619–629 (2013). https://doi.org/10.1007/s00606-012-0748-8

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  • DOI: https://doi.org/10.1007/s00606-012-0748-8

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