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Evaluation of meiotic abnormalities and pollen viability in aposporous and sexual tetraploid Paspalum notatum (Poaceae)

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Abstract

We analyzed anaphase I configurations and pollen viability in aposporous and sexual tetraploid (2n = 4x = 40) cytotypes of Paspalum notatum. Five natural aposporous accessions and three experimentally obtained sexual individuals were used. In addition, 16 (8 aposporous and 8 sexual) F1 hybrids, previously classified by their mode of reproduction, were analyzed. Cytogenetic observations revealed normal and abnormal anaphase I configurations in both aposporous and sexual genotypes. Anaphase I abnormalities were mainly laggard chromosomes, chromatin bridges, and micronuclei. On average, 44.36 % of aposporous meiocytes and 29.66 % of sexual ones showed abnormal anaphase I configurations. The total numbers of normal and abnormal anaphase I were highly significantly different between aposporous and sexual strains. The pollen viability test indicated that aposporous individuals had significantly more non-viable pollen than sexual ones; a positive correlation (r = 0.71; r 2 = 0.50) between the variables was detected. Analysis of aposporous and sexual hybrids confirmed differences in the numbers of normal and abnormal anaphase I patterns in the aposporous and sexual parents. However, similar proportions of viable pollen were produced by both groups of hybrids. In this case, the variables were not correlated (r = 0.23; r 2 = 0.05). Data from this study indicated that aposporous strains had a genetic rearrangement affecting meiosis that was absent in the experimentally obtained sexual individuals and that it was transmitted to the progeny. The possible association between meiotic abnormalities and the inheritance of apospory is discussed.

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Acknowledgments

The authors thank Florencia Galdeano for technical assistance. This study was financed by Agencia Nacional de Promoción Científica y Tecnológica, Argentina (PICT 2007 no. 00476); Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina (PIP 112-200801-01378), and Universidad Nacional de Rosario (AGR189). M. Podio, L.A. Siena, and D. Hojsgaard received fellowships from CONICET. C.L. Quarin and J.P. A. Ortiz are career members of CONICET.

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Authors and Affiliations

  1. Laboratorio de Biología Molecular, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Campo Experimental J. Villarino CC 14, S2125ZAA, Zavalla, Santa Fe, Argentina

    Maricel Podio, Lorena A. Siena, Juliana Stein & Juan Pablo A. Ortiz

  2. Instituto de Botánica del Nordeste (IBONE-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, CC209, 3400, Corrientes, Argentina

    Maricel Podio, Camilo L. Quarin & Juan Pablo A. Ortiz

  3. Department of Systematic Botany, Albrecht-von-Haller Institute of Plant Sciences, Georg-August-University of Göttingen, Untere Karspüle 2, 37073, Göttingen, Germany

    Diego Hojsgaard

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  1. Maricel Podio
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  2. Lorena A. Siena
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  3. Diego Hojsgaard
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  4. Juliana Stein
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  5. Camilo L. Quarin
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  6. Juan Pablo A. Ortiz
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Correspondence to Juan Pablo A. Ortiz.

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Podio, M., Siena, L.A., Hojsgaard, D. et al. Evaluation of meiotic abnormalities and pollen viability in aposporous and sexual tetraploid Paspalum notatum (Poaceae). Plant Syst Evol 298, 1625–1633 (2012). https://doi.org/10.1007/s00606-012-0664-y

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  • DOI: https://doi.org/10.1007/s00606-012-0664-y

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