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Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

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Abstract

Interspecific hybrids can be studied using methodologies in which the male gamete with high reproduction potential, viability, and fertility is prioritized. Passiflora species, with lush, showy, and exotic colors, have great potential for the ornamental plant market. In addition, artificial Passiflora hybrids were developed without many difficulties because of weak reproductive barriers. Thus, meiotic and post-meiotic behaviors were analyzed with 2% acetic carmine staining. Confirmation of interspecific hybridization was performed using SSR markers and GISH technique was used to detect genomic differentiation. The pollen viability of the parental and hybrids genotypes was tested using Alexander`s solution, fluorescein diacetate and in vitro germination tests were performed using culture medium. The meiotic behavior was regular and displayed haploid number n = 9 with nine bivalent (II) chromosomal, and pairing in 90% of the cells in diakinesis. There was a significant difference (p < 0.05) in terminal and interstitial chiasma frequencies. Meiotic irregularities observed were as follows: early and/or delayed chromosomes, disorientation of spindle fibers, transverse spindles, tripolar spindles, and asynchrony; and consequently irregular post-meiotic products were observed: monads, dyads, triads, and polyads. GISH was used in the interspecific hybrids and pairing between homeologous chromosomes, and bivalent and tetravalent formation were observed. From this study, we could conclude that hybrid genotypes are fertile and pollen grains are viable and can be used in breeding programs.

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Acknowledgements

The authors would like to thank UESC (Universidade Estadual de Santa Cruz), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and CAPES (Coordenação de Aperfeiçoamento de Pesssoal de Nível Superior) for the financial support and scholarships.

Funding

The research was supported by Universidade Estadual de Santa Cruz with financial resources; by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) with the scholarship given for the first and third authors; CNPq for the research grant given for the second author.

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  1. Laboratório de Melhoramento de Plantas (LAMEP), Departamento de Ciências Biológicas (DCB), Universidade Estadual de Santa Cruz (UESC), Rod. Jorge Amado, Km 16, Ilhéus, Bahia, 45662-900, Brazil

    Analu Cruz Souza, Margarete Magalhães Souza, Gonçalo Santos Silva, Manuella Palmeira Lavinscky & Viviane de Oliveira Souza

  2. Instituto de Ciências Agrárias e Sustentabilidade, Universidade Estadual de Goiás (UEG), Campus Nordeste, Unidade Posse, Av. Sra. de Santana, 598 - St. Santa Luzia, Posse, GO, 73900-000, Brazil

    Jôsie Cloviane de Oliveira Freitas

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  1. Analu Cruz Souza
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Analu Cruz Souza: Conducting laboratory experiment, data analysis, article writing. Margarete Magalhães Souza: Assistance and collaboration in all stages of the research. Gonçalo Santos Silva: Assistance in laboratory experiment and article writing. Manuella Palmeira Lavinscky: Assistance in laboratory. Viviane de Oliveira Souza: Assistance in laboratory. Jôsie Cloviane de Oliveira Freitas: Assistance in data analysis.

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Correspondence to Margarete Magalhães Souza.

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Souza, A.C., Souza, M.M., Silva, G.S. et al. Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii). Euphytica 219, 2 (2023). https://doi.org/10.1007/s10681-022-03110-z

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