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Genome size in Dipsacaceae and Morina longifolia (Morinaceae)

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Abstract

Dipsacaceae and Morinaceae have for a long time been regarded as separate but related families, whereas according to APG III they are included within the larger family Caprifoliaceae. Although genome size studies seldom provide conclusive characters for higher level systematics, they can yield useful information at a lower taxonomy level. In this study, we used DNA flow cytometry (supplemented by Feulgen densitometry) for measurement of genome size variation in the Dipsacaceae genera Cephalaria, Dipsacus, Knautia, Lomelosia, Pterocephalus, Scabiosa, Sixalix, Succisa, and Succisella, and Morina of the Morinaceae. At the monoploid level the Dipsacaceae genera (x = 7–10) vary 5.94-fold between 0.902 and 5.362 pg DNA (1Cx-value), whereas Morina longifolia (x = 17) has only 0.670 pg DNA. At the holoploid level 11.58-fold variation occurs between 0.902 and 10.446 pg DNA (1C-value). In Knautia sect. Trichera ploidy levels 2x, 4x, 6x are accompanied by corresponding increments of C-values, but genome downsizing is observed. In Knautia sect. Tricheroides the only investigated species K. integrifolia (2n = 20) has only 0.60-fold the mean genome size of sect. Trichera. Scabiosa canescens (2n = 2x = 16) has approximately double the C-value of all other Austrian Scabiosa species at the diploid level (pseudopolyploidy). These values raise concern against DNA-ploidy estimations at the interspecific level when chromosome numbers are unknown. The species sorted into two major clades of an existing phylogenetic tree of Dipsacaceae differ characteristically in their range of Cx-values. The Knautia–Cephalaria–Dipsacus–Succisella clade has the great majority of its Cx-values larger than those of the Scabiosa–Pterocephalus–Lomelosia clade.

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Acknowledgments

The authors thank Friedrich Ehrendorfer, Luise Schratt-Ehrendorfer, Elvira Hörandl, Veronika Mayer, Peter Schönswetter, Birgit Taumberger, and Karin Tremetsberger for providing material, and Dessislava Dimitrova, Ana Petrova, and Vladimir Vladimirov for helping with the Bulgarian species collection and identification. Thanks also to J. Doležel for standard seeds of S. cereale, H. vulgare and V. faba.

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  1. Department of Systematic and Evolutionary Botany, University of Vienna, Rennweg 14, 1030, Vienna, Austria

    Eva M. Temsch & Johann Greilhuber

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  1. Eva M. Temsch
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  2. Johann Greilhuber
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Correspondence to Eva M. Temsch.

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Temsch, E.M., Greilhuber, J. Genome size in Dipsacaceae and Morina longifolia (Morinaceae). Plant Syst Evol 289, 45–56 (2010). https://doi.org/10.1007/s00606-010-0330-1

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