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Gold nanoparticle-graphene quantum dots nanozyme for the wide range and sensitive electrochemical determination of quercetin in plasma droplets

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Abstract

A gold nanoparticle/graphene quantum dots (AuNP/GQD) nanozyme–modified screen-printed carbon electrode (SPCE) was developed for fast and ultrasensitive determination of quercetin by square-wave voltammetry. The nanosensing device was inserted in miniaturized equipment. Under the optimal experimental conditions, a good linear relationship between oxidation peak current and concentration of quercetin within a very wide range of 1.0 × 10−10 to 1.0 × 10−3 mol L−1 was obtained, with a very low limit of detection of 3.3 × 10−11 mol L−1. To prove the performance of the method, the reproducibility was evaluated and the RSD values were lower than 5.3% and 5.1% for the intra-day and inter-day measurements, respectively. The method was applied to the sensitive determination of quercetin in human plasma droplets with recovery rates of 92.6 to 101.7%.

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Acknowledgments

The authors are grateful to Dr. Ludmila Aricov for performing UV-Vis measurements.

Funding

This work was financially supported by UEFISCDI-CNCS, subordinated to The Ministry for Education and Research (MER), under grant numbers PN-III-P1-1.1-PD-2016-0190 and PN-III-P4-ID-PCE-2016-0050, PN 1916-Advanced Research in Micro/Nano-Electronics, Photonics and Micro/Nanobiosystems for Development of Applications in Intelligent Specialization Areas (MICRO-NANO-SIS PLUS), within PNCDI III.

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Authors and Affiliations

  1. Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Street, 060021, Bucharest, Romania

    Cristina Stefanov, Catalina Cioates Negut, Livia Alexandra Dinu Gugoasa & Jacobus (Koos) Frederick van Staden

  2. National Institute for Research and Development in Microtechnologies (IMT Bucharest), 126A Erou Iancu Nicolae Street, 077190, Voluntari, Ilfov, Romania

    Livia Alexandra Dinu Gugoasa

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  1. Cristina Stefanov
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  2. Catalina Cioates Negut
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Correspondence to Livia Alexandra Dinu Gugoasa.

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Stefanov, C., Negut, C.C., Gugoasa, L.A.D. et al. Gold nanoparticle-graphene quantum dots nanozyme for the wide range and sensitive electrochemical determination of quercetin in plasma droplets. Microchim Acta 187, 611 (2020). https://doi.org/10.1007/s00604-020-04587-y

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