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Non-conjugated polymer carbon dots for fluorometric determination of metronidazole

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Abstract

Non-conjugated polymer carbon dots (PCDs) with a 9% fluorescence quantum yield were synthesized by a pyrolytic method using polyethyleneimine as the sole precursor. The PCDs have an average size about 2.1 nm and a blue fluorescence, with excitation/emission maxima at 380/457 nm, that is quenched by the drug metronidazole. The method has a linear response in the 0.06–15 μg mL−1 metronidazole concentration range and a 20 ng mL−1 detection limit. Milk samples were spiked at two levels (0.6 and 5.0 μg mL−1), and the recoveries of metronidazole are in the range of 96.7–102.2%.

Schematic representation of preparation of non-conjugated polymer carbon dots (PCDs) and detection of metronidazole. Metronidazole with negative charge is easy to produce electrostatic interaction with polyethyleneimine chain with positive charge, which leads to PCDs fluorescence quenching, so as to realize metronidazole detection.

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Acknowledgements

This work received the support of the Development Program of the Ministry of Science and Technology of Jilin Province, China (Grant number 20180201011GX).

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Author notes

  1. Shuliang Yang and Long Wang contributed equally to this work.

Authors and Affiliations

  1. College of Chemistry, Jilin University, Changchun, 130012, China

    Shuliang Yang, Huiyu Li & Lan Ding

  2. Shenyang Academy of Environmental Sciences, Liaoning Provincial Key Laboratory for Urban Ecology, Shenyang, 110167, China

    Long Wang

  3. Department of Ophthalmology, the Second Hospital of Jilin University, Changchun, 130012, China

    Ling Zuo

  4. State Key Laboratory on Integrated Optoelectronics, College of Electronic Science & Engineering, Jilin University, Changchun, 130012, China

    Chun Zhao

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  1. Shuliang Yang
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  2. Long Wang
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  3. Ling Zuo
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  4. Chun Zhao
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  5. Huiyu Li
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  6. Lan Ding
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The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript. ‡These authors contributed equally.

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Correspondence to Huiyu Li or Lan Ding.

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Yang, S., Wang, L., Zuo, L. et al. Non-conjugated polymer carbon dots for fluorometric determination of metronidazole. Microchim Acta 186, 652 (2019). https://doi.org/10.1007/s00604-019-3746-5

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