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Detection of oxytetracycline in milk using a novel carbon dots-based fluorescence probe via facile pyrolysis synthesis

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Abstract

Amphiphilic blue-fluorescence carbon dots (B-CDs) were synthesized via pyrolysis method with citric acid and oleamine as precursors. B-CDs are monodispersed in ethanol, toluene, and ultrapure water with the average particle sizes of 3.33 nm, 2.05 nm, and 4.12 nm, respectively. The maximum emission wavelength of the B-CDs excitation at 370 nm is located at 459 nm. The B-CDs have good optical properties with excellent photostability. The fluorescence quantum yield (QY) of the as-prepared CDs is as high as 30.17%. The fluorescence of B-CDs is quenched because of static quenching by oxytetracycline. A high selective and sensitive fluorescence probe for detecting oxytetracycline was constructed with a linear range of 1.52–27.60 µg/mL and the detection limit of 0.33 µg/mL. The B-CDs-based fluorescence probe can be applied to analyze oxytetracycline in milk; the recoveries and relative standard are satisfactory. Furthermore, the B-CDs were exploited for imaging of SH-SY5Y cells. The results demonstrate that as-synthesized CDs can serve as a cellular imaging reagent owing to remarkable bioimaging performance. This work provides a new strategy for the detection of oxytetracycline in food.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request. Correspondence and requests for materials should be addressed to Chuan Dong.

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Funding

This study received financial supports from the Shanxi Scholarship Council of China (Nos. 2020–002 and 2021–014).

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Authors and Affiliations

  1. Institute of Environmental Science, Shanxi University, Wucheng Road 92#, Taiyuan, 030006, Shanxi Province, China

    Zhenhua Yang, Xintong Yang, Yuexia Zhang, Xiaopeng Fan, Yujuan Cao, Zhongping Li & Chuan Dong

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  1. Zhenhua Yang
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  2. Xintong Yang
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  3. Yuexia Zhang
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  4. Xiaopeng Fan
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  5. Yujuan Cao
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  6. Zhongping Li
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  7. Chuan Dong
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Contributions

Zhenhua Yang and Yuexia Zhang conceptualized the study. Xintong Yang conducted the experiment. Xiaopeng Fan and Yujuan Cao analyzed the data. Zhenhua Yang wrote the original manuscript. Yuexia Zhang, Zhongping Li, and Chuan Dong revised the manuscript. All authors read and approved the manuscript.

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Correspondence to Chuan Dong.

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Yang, Z., Yang, X., Zhang, Y. et al. Detection of oxytetracycline in milk using a novel carbon dots-based fluorescence probe via facile pyrolysis synthesis. Environ Sci Pollut Res 30, 84002–84010 (2023). https://doi.org/10.1007/s11356-023-28380-5

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  • DOI: https://doi.org/10.1007/s11356-023-28380-5

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