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Gold nanoparticle-based detection of dopamine based on fluorescence resonance energy transfer between a 4-(4-dialkylaminostyryl)pyridinium derived fluorophore and citrate-capped gold nanoparticles

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Abstract

A colorimetric/fluorometric dual-signal assay is described for the determination of dopamine (DA). A nanoprobe was obtained by linking a 4-(4-dialkylaminostyryl)pyridinium derived fluorophore to citrate-capped gold nanoparticles (AuNPs). The fluorescence of the fluorophore is quenched by the AuNPs via fluorescence resonance energy transfe. In the presence of DA, the catechol group of DA can absorb on the surface of AuNPs to induce aggregation, which is accompanied by a color change from red to blue. The yellow fluorescence of the fluorophore with excitation/emission maximum at 365/570 nm is recovered. The dual-signal detection allows the quantitative analysis of DA within 300 μM by the colorimetric method and 80 μM by the fluorometric method. The detection limits for the colorimetric/fluorometric methods are 1.85 μM and 0.29 μM, respectively. Quantitative determination of DA in spiked urine samples was successfully demonstrated, with recoveries ranging from 98.2 to 106.0%.

A colorimetric/fluorometric dual-signal assay is described for the determination of dopamine by linking a fluorophore to gold nanoparticles. The dopamine causes aggregation of the nanoparticles to induce color change, which is followed by the recovery of the fluorescence.

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Acknowledgments

We sincerely acknowledge the financial support of the National Natural Science Foundation of China (81701766 and 81702998).

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Authors and Affiliations

  1. State Key Laboratory of Natural Medicines, School of Basic Medical Sciences and Clinical Pharmacy, China Pharmaceutical University, Nanjing, 211198, Jiangsu, China

    Juanjuan Peng, Yang Zhong, Yaoquan Su & Lingzhi Zhao

  2. School of Materials Science and Engineering, Hebei Provincial Key Laboratory of Traffic Engineering Materials, Shijiazhuang Tiedao University, Shijiazhuang, 050043, China

    Na Zhou

  3. Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, 37673, South Korea

    Young-Tae Chang

  4. Department of Chemistry, Pohang University of Science and Technology, Pohang, Gyeongbuk, 37673, South Korea

    Young-Tae Chang

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  1. Juanjuan Peng
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  2. Na Zhou
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  3. Yang Zhong
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  4. Yaoquan Su
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  5. Lingzhi Zhao
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Correspondence to Juanjuan Peng, Lingzhi Zhao or Young-Tae Chang.

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Peng, J., Zhou, N., Zhong, Y. et al. Gold nanoparticle-based detection of dopamine based on fluorescence resonance energy transfer between a 4-(4-dialkylaminostyryl)pyridinium derived fluorophore and citrate-capped gold nanoparticles. Microchim Acta 186, 618 (2019). https://doi.org/10.1007/s00604-019-3727-8

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