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Nonenzymatic sensing of hydrogen peroxide using a glassy carbon electrode modified with graphene oxide, a polyamidoamine dendrimer, and with polyaniline deposited by the Fenton reaction

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Abstract

A highly sensitive electrochemical sensor is described for the determination of H2O2. It is based on based on the use of polyaniline that was generated in-situ and within 1 min on a glassy carbon electrode (GCE) with the aid of the Fe(II)/H2O2 system. Initially, a 2-dimensional composite was prepared from graphene oxide and polyamidoamine dendrimer through covalent interaction. It was employed as a carrier for Fe(II) ions. Then, the nanocomposite was drop-coated onto the surface of the GCE. When exposed to H2O2, the Fe(II) on the GCE is converted to Fe(III), and free hydroxy radicals are formed. The Fe(III) ions and the hydroxy radicals catalyze the oxidation of aniline to produce electroactive polyaniline on the GCE. The resulting sensor, best operated at a working potential as low as 50 mV (vs. SCE) which excludes interference by dissolved oxygen, has a linear response in the 500 nM to 2 mM H2O2 concentration range, and the detection limit is 180 nM. The sensor was successfully applied to the determination of H2O2 in spiked milk and fetal bovine serum samples.

Schematic presentation of a sensitive electrochemical sensor employed for detection of H2O2 in sophisticated matrices by using graphene oxide-PAMAM dendrimer as initiator container and Fe2+/H2O2 system as signal enhancer.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (21864013, 21505060), the Nature Science Foundation of Jiangxi Province (20161BAB213073), the Foundation of Jiangxi Educational Committee (GJJ150327), the Natural Science Foundation of Fujian Province (2018 J01442), the Fujian Educational Committee (JAT170487), the Foundation for High-Level Talents in Quanzhou City (2017Z029), Scientific Research Foundation of Jiangxi Normal University, the Open Project Program of Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University (No. KLFS-KF-201710), Outstanding Youth Funds of Jiangxi Normal University.

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Authors and Affiliations

  1. Ministry of Education Key Laboratory of Functional Small Organic Molecule, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, People’s Republic of China

    Juan Tang, Lulu Huang, Yu Cheng & Ping Li

  2. College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou, 362000, Fujian Province, People’s Republic of China

    Junyang Zhuang

  3. Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, 350108, People’s Republic of China

    Dianping Tang

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  1. Juan Tang
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Tang, J., Huang, L., Cheng, Y. et al. Nonenzymatic sensing of hydrogen peroxide using a glassy carbon electrode modified with graphene oxide, a polyamidoamine dendrimer, and with polyaniline deposited by the Fenton reaction. Microchim Acta 185, 569 (2018). https://doi.org/10.1007/s00604-018-3089-7

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