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An impedimetric aptasensor for Shigella dysenteriae using a gold nanoparticle-modified glassy carbon electrode

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Abstract

This work describes an aptasensor for the foodborne pathogen Shigella dysenteriae (S. dysenteriae). A glassy carbon electrode (GCE) was modified with gold nanoparticles (AuNPs) by electrodeposition. Then, thiolated aptamer for S. dysenteriae detection was self-assembled on the surface of the modified GCE, and any free residual AuNPs were blocked with 6-mercapto-1-hexanol. The size, morphology, and distribution of the AuNPs were characterized by field emission scanning electron microscopy. Detection of S. dysenteriae was performed measurement of the charge transfer resistance (Rct) before and after addition of S. dysenteriae using hexacyanoferrate as an electrochemical probe. The interaction between the aptamer and outer-membrane proteins of S. dysenteriae lead to an increase in the Rct of the sensor. The assay has a linear dynamic range that extends from 101 to 106 CFU.mL−1 and a limit of detection of 100 CFU.mL−1. It can differentiate between alive S. dysenteriae and other pathogens. Dead S. dysenteriae cells do not have any effect on selectivity. Unpasteurized and pasteurized skim milk and some water samples were spiked with S. dysenteriae and then successfully examined by this method. The results were validated by real-time PCR. The method is fast, low-cost, highly sensitive, and specific. Hence, it represents a valuable tool in food quality control.

Schematic presentation of a label free impedimetric aptasensor for Shigella dysenteriae using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and 6-mercapto-1-hexanol (MCH). The limit of detection of this aptasensor is as low as 1 CFU.mL−1 for target bacteria.

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References

  1. The HC, Thanh DP, Holt KE, Thomson NR, Baker S (2016) The genomic signatures of Shigella evolution, adaptation and geographical spread. Nat Rev Microbiol 14:235–250. https://doi.org/10.1038/nrmicro.2016.10

    Article  CAS  PubMed  Google Scholar 

  2. Duan N, Ding X, Wu S, Xia Y, Ma X, Wang Z, Chen J (2013) In vitro selection of a DNA aptamer targeted against Shigella dysenteriae. J Microbiol Methods 94:170–174. https://doi.org/10.1016/j.mimet.2013.06.016

    Article  CAS  PubMed  Google Scholar 

  3. WHO (2017) Guidelines for Drinking-water Quality, fourth. Geneva

  4. FDA (2018) Health Educators - Food Safety for Moms-to-Be: Medical Professionals - Foodborne Pathogens. Center for Food Safety and Applied Nutrition. https://www.fda.gov/food/resourcesforyou/healtheducators/ucm091681.htm. Accessed 19 october 2007

  5. Bagheryan Z, Raoof J-B, Golabi M, Turner APF, Beni V (2016) Diazonium-based impedimetric aptasensor for the rapid label-free detection of Salmonella typhimurium in food sample. Biosens Bioelectron 80:566–573. https://doi.org/10.1016/j.bios.2016.02.024

    Article  CAS  PubMed  Google Scholar 

  6. Izadi Z, Sheikh-Zeinoddin M, Ensafi AA, Soleimanian-Zad S (2016) Fabrication of an electrochemical DNA-based biosensor for Bacillus cereus detection in milk and infant formula. Biosens Bioelectron 80:582–589. https://doi.org/10.1016/j.bios.2016.02.032

    Article  CAS  PubMed  Google Scholar 

  7. Zelada-Guillen GA, Riu J, Düzgün A, Rius FX (2009) Immediate detection of living bacteria at ultralow concentrations using a carbon nanotube based potentiometrie aptasensor. Angew Chemie - Int Ed 48:7334–7337. https://doi.org/10.1002/anie.200902090

    Article  CAS  Google Scholar 

  8. Li L, Yuan Y, Chen Y, Zhang P, Bai Y, Bai L (2018) Aptamer based voltammetric biosensor for Mycobacterium tuberculosis antigen ESAT-6 using a nanohybrid material composed of reduced graphene oxide and a metal-organic framework. Microchim Acta 185:379. https://doi.org/10.1007/s00604-018-2884-5

    Article  CAS  Google Scholar 

  9. Templier V, Roux A, Roupioz Y, Livache T (2016) Ligands for label-free detection of whole bacteria on biosensors: a review. TrAC Trends Anal Chem 79:71–79. https://doi.org/10.1016/j.trac.2015.10.015

    Article  CAS  Google Scholar 

  10. Liang G, Man Y, Jin X, Pan L, Liu X (2016) Aptamer-based biosensor for label-free detection of ethanolamine by electrochemical impedance spectroscopy. Anal Chim Acta 936:222–228. https://doi.org/10.1016/j.aca.2016.06.056

    Article  CAS  PubMed  Google Scholar 

  11. Rezaei B, Jamei HR, Ensafi AA (2018) Lysozyme aptasensor based on a glassy carbon electrode modified with a nanocomposite consisting of multi-walled carbon nanotubes, poly(diallyl dimethyl ammonium chloride) and carbon quantum dots. Microchim Acta 185:180. https://doi.org/10.1007/s00604-017-2656-7

    Article  CAS  Google Scholar 

  12. Jia F, Duan N, Wu S, Ma X, Xia Y, Wang Z, Wei X (2014) Impedimetric aptasensor for Staphylococcus aureus based on nanocomposite prepared from reduced graphene oxide and gold nanoparticles. Microchim Acta 181:967–974. https://doi.org/10.1007/s00604-014-1195-8

    Article  CAS  Google Scholar 

  13. Kaur H, Shorie M, Sharma M, Ganguli AK, Sabherwal P (2017) Bridged rebar graphene functionalized aptasensor for pathogenic E. coli O78:K80:H11 detection. Biosens Bioelectron 98:486–493. https://doi.org/10.1016/j.bios.2017.07.004

    Article  CAS  PubMed  Google Scholar 

  14. Roushani M, Shahdost-Fard F (2015) Fabrication of an ultrasensitive ibuprofen nanoaptasensor based on covalent attachment of aptamer to electrochemically deposited gold-nanoparticles on glassy carbon electrode. Talanta 144:510–516. https://doi.org/10.1016/j.talanta.2015.06.052

    Article  CAS  PubMed  Google Scholar 

  15. Ma Y, Liu J, Li H (2017) Diamond-based electrochemical aptasensor realizing a femtomolar detection limit of bisphenol a. Biosens Bioelectron 92:21–25. https://doi.org/10.1016/j.bios.2017.01.041

    Article  CAS  PubMed  Google Scholar 

  16. Heydari-Bafrooei E, Amini M, Saeednia S (2017) Electrochemical detection of DNA damage induced by bleomycin in the presence of metal ions. J Electroanal Chem 803:104–110. https://doi.org/10.1016/j.jelechem.2017.09.031

    Article  CAS  Google Scholar 

  17. Labib M, Zamay AS, Kolovskaya OS, Reshetneva IT, Zamay GS, Kibbee RJ, Sattar SA, Zamay TN, Berezovski MV (2012) Aptamer-based viability impedimetric sensor for bacteria. Anal Chem 84:8966–8969. https://doi.org/10.1021/ac302902s

    Article  CAS  PubMed  Google Scholar 

  18. Liu PY, Chin LK, Ser W, Ayi TC, Yap PH, Bourouina T, Leprince-Wang Y (2014) An optofluidic imaging system to measure the biophysical signature of single waterborne bacteria. Lab Chip 14:4237–4243. https://doi.org/10.1039/C4LC00783B

    Article  CAS  PubMed  Google Scholar 

  19. Sheikhzadeh E, Chamsaz M, Turner APF, Jager EWH, Beni V (2016) Label-free impedimetric biosensor for Salmonella Typhimurium detection based on poly [pyrrole-co-3-carboxyl-pyrrole] copolymer supported aptamer. Biosens Bioelectron 80:194–200. https://doi.org/10.1016/j.bios.2016.01.057

    Article  CAS  PubMed  Google Scholar 

  20. Jia F, Duan N, Wu S, Dai R, Wang Z, Li X (2016) Impedimetric Salmonella aptasensor using a glassy carbon electrode modified with an electrodeposited composite consisting of reduced graphene oxide and carbon nanotubes. Microchim Acta 183:337–344. https://doi.org/10.1007/s00604-015-1649-7

    Article  CAS  Google Scholar 

  21. Luo J, Wang J, Mathew AS, Yau ST (2016) Ultrasensitive detection of Shigella species in blood and stool. Anal Chem 88:2010–2014. https://doi.org/10.1021/acs.analchem.5b04242

    Article  CAS  PubMed  Google Scholar 

  22. Ma K, Deng Y, Bai Y, Xu D, Chen E, Wu H, Li B, Gao L (2014) Rapid and simultaneous detection of Salmonella, Shigella, and Staphylococcus aureus in fresh pork using a multiplex real-time PCR assay based on immunomagnetic separation. Food Control 42:87–93. https://doi.org/10.1016/j.foodcont.2014.01.042

    Article  CAS  Google Scholar 

  23. Tang X-J, Yang Z, Chen X-B, Tian W-F, Tu C-N, Wang H-B (2018) Verification and large scale clinical evaluation of a national standard protocol for Salmonella spp ./Shigella spp. screening using real-time PCR combined with guided culture. J Microbiol Methods 145:14–19. https://doi.org/10.1016/j.mimet.2017.12.007

    Article  CAS  PubMed  Google Scholar 

  24. Kumar S, Balakrishna K, Batra HV (2008) Enrichment-ELISA for detection of Salmonella typhi from food and water samples. Biomed Environ Sci 21:137–143. https://doi.org/10.1016/S0895-3988(08)60019-7

    Article  CAS  PubMed  Google Scholar 

  25. Febo TD, Schirone M, Visciano P, Portanti O, Armillotta G, Persiani T, Giannatale E, Tittarelli M, Luciani M (2018) Development of a capture ELISA for rapid detection of Salmonella enterica in food samples. Food Anal Methods 2018. https://doi.org/10.1007/s12161-018-1363-2

  26. Shan S, Liu D, Guo Q, Wu S, Chen R, Luo K, Hu L, Xiong Y, Lai W (2016) Sensitive detection of Escherichia coli O157:H7 based on cascade signal amplification in ELISA. J Dairy Sci 99:7025–7032. https://doi.org/10.3168/jds.2016-11320

    Article  CAS  PubMed  Google Scholar 

  27. Li Y, Cao L, Zhang C, Chen Q, Lu F, Bie X, Lu Z (2013) Development and evaluation of a PCR-ELISA assay for the detection and quanti fi cation of Cronobacter spp. Int Dairy J 33:27–33. https://doi.org/10.1016/j.idairyj.2013.06.009

    Article  CAS  Google Scholar 

  28. Ojha SC, Yean Yean C, Ismail A, Banga Singh KK (2013) A pentaplex PCR assay for the detection and differentiation of shigella species. Biomed Res Int 2013:412370. https://doi.org/10.1155/2013/412370

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Zhong QP, Wang L, Wang B, Chen HY (2011) Loop-mediated isothermal amplification method for rapid detection of Shigella dysenteriae. Appl Mech Mater 140:369–373. https://doi.org/10.4028/www.scientific.net/AMM.140.369

    Article  CAS  Google Scholar 

  30. Du M, Li J, Zhao R, Yang Y, Wang Y, Ma K, Cheng X, Wan Y, Wu X (2018) Effective pre-treatment technique based on immune-magnetic separation for rapid detection of trace levels of Salmonella in milk. Food Control 91:92–99. https://doi.org/10.1016/j.foodcont.2018.03.032

    Article  CAS  Google Scholar 

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Acknowledgements

Authors wish to thank the Research Institute of Biotechnology and Bioengineering for supporting this study.

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Authors and Affiliations

  1. Department of Food Science and Technology, Faculty of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran

    Seyed Sanaan Zarei & Sabihe Soleimanian-Zad

  2. Research Institute of Biotechnology and Bioengineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran

    Sabihe Soleimanian-Zad

  3. Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, Iran

    Ali A. Ensafi

Authors
  1. Seyed Sanaan Zarei
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  2. Sabihe Soleimanian-Zad
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  3. Ali A. Ensafi
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Correspondence to Sabihe Soleimanian-Zad or Ali A. Ensafi.

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Zarei, S.S., Soleimanian-Zad, S. & Ensafi, A.A. An impedimetric aptasensor for Shigella dysenteriae using a gold nanoparticle-modified glassy carbon electrode. Microchim Acta 185, 538 (2018). https://doi.org/10.1007/s00604-018-3075-0

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