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Aptamer based voltammetric biosensor for Mycobacterium tuberculosis antigen ESAT-6 using a nanohybrid material composed of reduced graphene oxide and a metal-organic framework

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Abstract

The 6-kDa early secretory antigenic target referred to as ESAT-6 is a virulence factor secreted by Mycobacterium tuberculosis (MTB). This work describes a voltammetric aptasensor for ultrasensitive detection of ESAT-6. Reduced graphene oxide doped with metal-organic framework (MOF-rGO) was deposited on a glassy carbon electrode (GCE). This increases the immobilization of electroactive Toluidine Blue (TB) and facilitates the electron transfer from TB to the modified GCE. Platinum/gold core/shell (Pt@Au) nanoparticles were used to assemble thiolated ESAT-6 binding aptamer (EBA) on a modified electrode and to further amplify the response to TB. The modified GCE, typically operated at −0.36 V (vs. SCE), has a linear response in 1.0 × 10−4 to 2.0 × 102 ng⋅mL-1 ESAT-6 concentration range, and the limit of detection (LOD) for ESAT-6 is as low as 3.3 × 10−5 ng⋅mL-1. It exhibits satisfactory specificity and reproducibility when analyzing spiked human serum.

Schematic presentation of a voltammetric aptasensor for Mycobacterium tuberculosis antigen ESAT-6 using a glassy carbon electrode modified with reduced graphene oxide (rGO) and a metal-organic framework (MOF). The limit of detection for ESAT-6 is as low as 3.3 × 10−5 ng/mL.

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Acknowledgements

This work is supported by National Natural Science Foundation of China (81601856), the Third Batch of Young Backbone Teachers Funding Program in Colleges and Universities of Chongqing City ([2016] No. 53), Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City (cstc2014kjrc-qnrc00004) and Fundamental and Advanced Research Projects of Chongqing City (cstc2016jcyjA0275).

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  1. Linlin Li and Yonghua Yuan contributed equally to this work.

Authors and Affiliations

  1. Engineering Technology Research Center for Pharmacodynamic Evaluation of Chongqing, College of Pharmacy, Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Linlin Li, Yonghua Yuan, Yongjie Chen, Pu Zhang, Yan Bai & Lijuan Bai

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  1. Linlin Li
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  2. Yonghua Yuan
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  3. Yongjie Chen
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  4. Pu Zhang
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  5. Yan Bai
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  6. Lijuan Bai
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Correspondence to Lijuan Bai.

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Li, L., Yuan, Y., Chen, Y. et al. Aptamer based voltammetric biosensor for Mycobacterium tuberculosis antigen ESAT-6 using a nanohybrid material composed of reduced graphene oxide and a metal-organic framework. Microchim Acta 185, 379 (2018). https://doi.org/10.1007/s00604-018-2884-5

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