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Visual and colorimetric determination of H2O2 and glucose based on citrate-promoted H2O2 sculpturing of silver nanoparticles

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Abstract

Isotropic silver nanoparticles (iAg NPs) can be easily prepared at low costs, have a low electrochemical potential and high extinction coefficient. An effective colorimetric assay for H2O2 is reported here based on the finding that H2O2 can induce the shape transformation of citrate-capped iAg NPs with the help of citrate. The substantial shape variation affords an apparent surface plasmon resonance (SPR) shift, accompanied by a vivid color change from light yellow to mauve. The color change can be observed visually if the concentration of H2O2 is 2 μM or higher. A good linear relationship was obtained over the concentration range of 0.2–32 μM with a limit of detection of 90 nM. By making use of glucose oxidase, the method is further extended to glucose detection. Glucose at a concentration as low as 10 μM can be well determined with bare eyes. Benefitting from the high selectivity, the detection of glucose in human serum is realized, and the results are in good agreement with those provided by a clinical analyzer.

Schematic presentation of a colorimetric assay for H2O2 and glucose based on citrate-promoted H2O2-mediated shape transformation of the isotropic silver nanoparticles (Ag NPs). The shape variation of isotropic Ag NPs induces a color change from light yellow to mauve.

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Acknowledgements

The authors are grateful to the Jiangsu Province Natural Science Foundation of China (BK20150228), the Natural Science Foundation of China (21601072), Natural Science Foundation of the Jiangsu Higher Education Institutions of China (16KJB510009).

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Authors and Affiliations

  1. School of Chemistry and Materials Science, Jiangsu Key Laboratory of Green Synthesis for Functional Materials, Jiangsu Normal University, Xuzhou, Jiangsu, People’s Republic of China, 221116

    Chenghua Zong, Bo Li, Jing Wang, Xiaojun Liu, Wenfeng Zhao, Qingquan Zhang & Yang Yu

  2. School of Physics and Electronic Engineering, Jiangsu Normal University, Xuzhou, 221116, People’s Republic of China

    Xinming Nie

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  1. Chenghua Zong
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  2. Bo Li
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Correspondence to Chenghua Zong or Yang Yu.

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The author state that the blood related experiments were performed in strict accordance with the WHO guidelines on blood drawing (WHO Publication ISBN-13: 978-92-4-159922-1, 2010) and was approved by Jiangsu Normal University. The authors also state that informed consent was obtained for any experimentation with human subjects and the Jiangsu Normal University is committed to the protection and safety of human subjects involved in research.

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Zong, C., Li, B., Wang, J. et al. Visual and colorimetric determination of H2O2 and glucose based on citrate-promoted H2O2 sculpturing of silver nanoparticles. Microchim Acta 185, 199 (2018). https://doi.org/10.1007/s00604-018-2737-2

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