Abstract
The authors describe a dual signal amplification strategy for improving the sensitivity of electrochemical aptasensor. Hydroxyapatite nanoparticles (HAP-NPs) serve as the support for deposition of the respective aptamer. Both the HAP-NPs and the aptamer contain phosphate groups which can react with molybdate to form a redox-active molybdophosphate precipitate on the surface of a glassy carbon electrode (GCE). On applying a relatively low voltage of 0.21 V (vs. Ag/AgCl), a current is generated whose intensity depends on the concentration of the analyte. The cancer biomarker platelet-derived growth factor BB (PDGF-BB) is chosen as a model antigen (analyte). The assay works by sequential deposition of antibody against PDGF-BB, analyte (PDGF-BB) and anti-PDGF-BB aptamer modified HAP-NPs on the GCE to form a sandwich structure. The amperometric signal is linear in the 0.1 pg.mL−1 to 10 ng.mL−1 PDGF-BB concentration range, with a detection limit as low as 50 fg.mL−1. The assay was successfully applied to the determination of PDGF-BB in serum samples. In our perception, this signal amplification strategy has a wide scope in that it can be adapted to the preparation of other aptasensors for biomarkers and related species.
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Acknowledgments
The authors thank the support of this work by the National Natural Science Foundation of China (No. 21575165), Tianjin Clinical Research Center for Organ Transplantation Grant (No. 15ZXLCSY00070) and Science and technology fund of Tianjin Municipal Health Bureau (No. 2013 KZ034).
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Jiang, W., Tian, D., Zhang, L. et al. Dual signal amplification strategy for amperometric aptasensing using hydroxyapatite nanoparticles. Application to the sensitive detection of the cancer biomarker platelet-derived growth factor BB. Microchim Acta 184, 4375–4381 (2017). https://doi.org/10.1007/s00604-017-2471-1
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DOI: https://doi.org/10.1007/s00604-017-2471-1