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Paper-based device with on-chip reagent storage for rapid extraction of DNA from biological samples

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Abstract

Conventional methods for extraction of DNA are expensive, time-consuming and tedious. To overcome these limitations, a paper-based DNA extraction device was developed that incorporates sponge-based buffer storage, a paper-based valve and channels of different length to autonomously direct the reagent and sample to the Fusion 5 disk for DNA capturing. With this device, DNA can be extracted within 2 min from only 30 μL samples of whole blood, serum, breast cancer cell, saliva, sputum and bacterial suspension. The device can also extract Hepatitis B Virus DNA from clinical blood samples and after quantification shows a detection limit as low as 104 copies⋅mL−1. This highlights its potential use in future diagnostics. The performance of the device was similar to that of the commercial QIAamp DNA micro kits and the FTA card. In our perception, this simple, inexpensive, portable and disposable device holds great promise in terms of POC testing in resource-poor settings.

Schematic of a paper-based DNA extraction device that incorporates sponge-based on-chip reagent storage, a paper-based valve and channels of different length to autonomously direct the reagent and sample to the Fusion 5 disk for DNA capturing.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (11472224, 11672246).

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Authors and Affiliations

  1. School of Life Sciences, Northwestern Polytechnical University, Xi’an, 710072, People’s Republic of China

    Ruihua Tang, Hui Yang & Qibing Mei

  2. Key Laboratory for Space Bioscience and Biotechnology, Northwestern Polytechnical University, Xi’an, 710072, People’s Republic of China

    Ruihua Tang, Hui Yang & Qibing Mei

  3. Bioinspired Engineering and Biomechanics Center (BEBC), Xi’an Jiaotong University, Xi’an, 710049, People’s Republic of China

    Ruihua Tang, Jane Ru Choi, Yan Gong, Jie Hu & Feng Xu

  4. The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, 710049, People’s Republic of China

    Jane Ru Choi, Yan Gong, Jie Hu & Feng Xu

  5. Xi’an Diandi Biotech Company, Xi’an, 710049, People’s Republic of China

    Ting Wen

  6. Department of Chemistry, University of Texas at El Paso, 500 West University Ave, El Paso, TX, 79968, USA

    XiuJun Li

  7. School of Finance and Economics, Xi’an Jiaotong University, Xi’an, People’s Republic of China

    Bo Xu

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  1. Ruihua Tang
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  2. Hui Yang
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Correspondence to Hui Yang or Feng Xu.

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Tang, R., Yang, H., Choi, J.R. et al. Paper-based device with on-chip reagent storage for rapid extraction of DNA from biological samples. Microchim Acta 184, 2141–2150 (2017). https://doi.org/10.1007/s00604-017-2225-0

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  • DOI: https://doi.org/10.1007/s00604-017-2225-0

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