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Paper-Based Nucleic Acid Detection for Point-of-Care Diagnostics

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Paper-Based Medical Diagnostic Devices

Part of the book series: Bioanalysis ((BIOANALYSIS,volume 10))

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Abstract

Nucleic acid detection has widely been used in key biosensing applications starting from diagnostics, food and water safety, and forensics. Traditionally operated in the laboratory, it has become an essential method to detect nucleic acids sensitively and reliably. With the increasing need of performing the nucleic acid detection outside of the traditional laboratory settings, the concept of “Lab-on-a-Chip”, colloquially known as microfluidic chips, has emerged to offer a portable platform for the field-based diagnostics. Using a fraction of the reagents and short travel length of fluids, it has enabled a rapid progress in point-of-care diagnostics. However, there are still formidable challenges lie ahead. One of the key bottlenecks for a wider acceptance of microfluidics in the main stream diagnostics has been the material of devices and the peripherals required to run the diagnostic device. Currently, polydimethylsiloxane (PDMS) material is a commonly used material for microfluidic applications. Despite its outstanding optical properties and easiness of molding and bonding, this material has intrinsically hydrophobic properties that require a pressure source to drive the sample fluid into the microchannel unless it has been treated prior to filling to become hydrophilic temporarily. The material cost is another prohibiting factor for a wide-spread use of PDMS.

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Authors and Affiliations

  1. Division of Engineering, New York University Abu Dhabi (NYUAD), P.O. Box 129188, Abu Dhabi, United Arab Emirates

    Jongmin Kim & Yong-Ak Song

  2. Department of Chemical and Biomolecular Engineering, Tandon School of Engineering, NYU, Brooklyn, NY, 11201, USA

    Yong-Ak Song

  3. Department of Biomedical Engineering, Tandon School of Engineering, NYU, Brooklyn, NY, 11201, USA

    Yong-Ak Song

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  1. Department of Electrical Engineering, Kwangwoon University, Seoul, Korea (Republic of)

    Jeong Hoon Lee

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Kim, J., Song, YA. (2021). Paper-Based Nucleic Acid Detection for Point-of-Care Diagnostics. In: Lee, J.H. (eds) Paper-Based Medical Diagnostic Devices. Bioanalysis, vol 10. Springer, Singapore. https://doi.org/10.1007/978-981-15-8723-8_5

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