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An enhanced SPR immunosensing platform for human IgG based on the use of silver nanocubes and carboxy-functionalized graphene oxide

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Abstract

The article describes a wavelength modulation surface plasmon resonance (SPR) biosensor for human IgG. The sensor is based on the use of silver nanocubes (SNCs) and carboxy-functionalized graphene oxide (cGO). The surface of the SNCs was modified with 3-mercaptopropinic acid by silver-thiol chemistry, and this protects the SNCs from oxidation. The cGO possesses abundant carboxy groups that allow high-density immobilization of anti-human IgG on the gold film of the SPR chip. Successful conjugation to the surface was demonstrated by X-ray photoelectron spectroscopy. The SPR biosensor responds to human IgG in the 0.075 to 40 μg mL-1 concentration range. The limit of quantification (LOQ) is smaller by factors of 32, 8 and 4 compared to (a) a conventional sensor, (b) a GO-based sensor, and (c) a cGO-based sensor. The detection scheme presented here offers a versatile and sensitive platform also for determination of other proteins if the corresponding receptors are available.

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Acknowledgments

This work was supported by Special-funded Program on National Key Scientific Instruments and Equipment Development of China (No. 2012YQ090194), National Natural Science Foundation of China (Nos. 21207047 and 21405057), and Science and Technology Developing Foundation of Jilin Province (Nos. 20150311099YY, 20121808 and 20150204017YY).

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Authors and Affiliations

  1. College of Chemistry, Jilin University, Qianjin Street 2699, Changchun, 130012, People’s Republic of China

    Qiong Wu, Daqian Song, Di Zhang & Ying Sun

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  1. Qiong Wu
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  2. Daqian Song
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  3. Di Zhang
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  4. Ying Sun
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Correspondence to Ying Sun.

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Wu, Q., Song, D., Zhang, D. et al. An enhanced SPR immunosensing platform for human IgG based on the use of silver nanocubes and carboxy-functionalized graphene oxide. Microchim Acta 183, 2177–2184 (2016). https://doi.org/10.1007/s00604-016-1853-0

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  • DOI: https://doi.org/10.1007/s00604-016-1853-0

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