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Sensitive detection of hydrogen peroxide in foodstuff using an organic–inorganic hybrid multilayer-functionalized graphene biosensing platform

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Abstract

We report on a new electrochemical biosensing strategy for the sensitive detection of hydrogen peroxide (H2O2) in foodstuff samples. It is based on a gold electrode modified with layer of graphene patterned with a multilayer made from an organic–inorganic hybrid nanomaterial. Initially, a layer of thionine (Th) was assembled on the surface of the graphene nanosheets, and these were then cast on the surface of the electrode for the alternate assembly of gold nanoparticles and horseradish peroxidase. The large surface-to-volume ratio and high conductivity of the nanosheets provides a benign microenvironment for the construction of the biosensor. The use of such a multilayer not only shortens the electron transfer pathway of the active center of the enzyme due to the presence of gold nanoparticles, but also enhances the electrocatalytic efficiency of the biosensor toward the reduction of H2O2. The electrochemical characteristics of the biosensor were studied by cyclic voltammetry and chronoamperometry. The number of layers, the operating potential, and the pH of the supporting electrolyte were optimized. Linear response is obtained for the range from 0.5 μM to 1.8 mM of H2O2, the detection limit is 10 nM (at S/N = 3), and 95% of the steady-state current is reached within 2 s. The method was applied to sense H2O2 in spiked sterilized milk and correlated excellently with the permanganate titration method.

A new electrochemical biosensing strategy for sensitive detection of hydrogen peroxide in foodstuff was developed by using a gold electrode modified with a layer of graphene nanosheets patterned with a multilayer made from an organic–inorganic hybrid nanomaterial.

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Acknowledgement

The support by the National Natural Science Foundation of China (21075019, 20735002), the Research Fund for the Doctoral Program of Higher Education of China (20103514120003), the “973” National Basic Research Program of China (2010CB732403), and Program for Returned High-Level Overseas Chinese Scholars of Fujian Province (XRC-0929) is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Chemistry, Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, 350108, People’s Republic of China

    Yuling Cui, Bing Zhang, Bingqian Liu, Huafeng Chen, Guonan Chen & Dianping Tang

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  1. Yuling Cui
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  2. Bing Zhang
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  3. Bingqian Liu
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  4. Huafeng Chen
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  5. Guonan Chen
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  6. Dianping Tang
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Correspondence to Dianping Tang.

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Cui, Y., Zhang, B., Liu, B. et al. Sensitive detection of hydrogen peroxide in foodstuff using an organic–inorganic hybrid multilayer-functionalized graphene biosensing platform. Microchim Acta 174, 137–144 (2011). https://doi.org/10.1007/s00604-011-0608-1

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  • DOI: https://doi.org/10.1007/s00604-011-0608-1

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