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Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer

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Abstract

We demonstrate an ultrasensitive time-resolved fluorescence immunoassay for bisphenol A (BPA) where gold nanoparticles (Au-NPs) were modified with anti-BPA antibody and thiolated dsDNA-biotin acting as a signal amplifier. In a competitive reaction, the analyte (BPA) competes with immobilized BPA-ovalbumin conjugate on the surface of microtiter plates to bind to the anti-BPA antibodies on the surface of the Au-NPs. In the next step, a Eu(III)-labeled streptavidin is added to link to the SH-dsDNA-biotin as a tracer. Fluorescence is amplified via both the Au-NPs and the biotin-streptavidin systems, and its intensity is measured in a time-resolved fluorescence immunoassay. The linear range is from 1.0 fg∙mL−1 to 1.0 ng∙mL−1, and the detection limit is 0.3 fg mL−1, respectively. The method was applied to the determination of BPA in spiked water sample, with recoveries ranging from 90.2 to 106.4 %.

A time-resolved fluorescence immunoassay based on the functionalized gold nanoparticles as signal amplifier was constructed for BPA detection.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 20877037).

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Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252059, China

    Lingyun Du, Chunyan Zhang, Longjun Wang, Yuanfu Zhang & Shuhao Wang

  2. College of Life Science, Liaocheng University, Liaocheng, 252059, China

    Guofu Liu

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  1. Lingyun Du
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  2. Chunyan Zhang
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  3. Longjun Wang
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  4. Guofu Liu
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  5. Yuanfu Zhang
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  6. Shuhao Wang
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Correspondence to Shuhao Wang.

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Du, L., Zhang, C., Wang, L. et al. Ultrasensitive time-resolved microplate fluorescence immunoassay for bisphenol A using a system composed on gold nanoparticles and a europium(III)-labeled streptavidin tracer. Microchim Acta 182, 539–545 (2015). https://doi.org/10.1007/s00604-014-1356-9

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  • DOI: https://doi.org/10.1007/s00604-014-1356-9

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