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Immunochromatographic lateral flow strip for on-site detection of bisphenol A

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Abstract

We have developed a lateral flow assay (LFA) for the detection of bisphenol A (BPA) in water samples. Antibody against BPA was labeled with gold nanoparticles, and these conjugates were used as the recognition probes for the construction of an LFA strip. The diameter of the gold nanoparticles, the amount of antibody, the pH of the buffer, and the categories of the conjugation pad were optimized. The resulting method has a (visual) detection limit of 5 ppb, and of 0.92 ppb if used in combination with professional software. This LFA displays excellent specificity and was applied to spiked water samples with satisfactory results.

Bisphenol A in water samples could be rapid and sensitively screened by the immunochramatographic lateral flow strip in less than 15 min. The limit of dectection was as low as 5 ppb and 0.92 ppb by naked-eye observation and software analysis, respectively, which meet the requirements of on-site and rapid detection of BPA in water samples.

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Acknowledgments

This work is financially supported by the Huangshan Young Scholar Fund of Hefei University of Technology (407–037025), the Science and Technology Research Project of General Administration of Quality Supervision, Inspection and Quarantine of P. R. China (201210127), the 12th Five Years Key Programs (2012BAK08B01, 2012BAK17B10, SS2012AA101001), and the Fundamental Research Funds for the Central Universities (2012HGCX0003).

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Authors and Affiliations

  1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, People’s Republic of China

    Zhanlong Mei, Yi Deng, Feng Xue, Youhao Zhong, Jingjing Wu, Huan Yang, Zhichao Wang, Lei Zheng & Wei Chen

  2. School of Food Science and Technology, Jiangnan University, Wuxi, 210004, People’s Republic of China

    Huaqin Chu

  3. Jiangsu Entry-exit Inspection and Quarantine Bureau, Nanjing, 200002, People’s Republic of China

    Feng Xue

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  1. Zhanlong Mei
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  2. Yi Deng
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  3. Huaqin Chu
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  4. Feng Xue
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  5. Youhao Zhong
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  6. Jingjing Wu
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  7. Huan Yang
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  8. Zhichao Wang
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  9. Lei Zheng
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  10. Wei Chen
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Correspondence to Wei Chen.

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Mei, Z., Deng, Y., Chu, H. et al. Immunochromatographic lateral flow strip for on-site detection of bisphenol A. Microchim Acta 180, 279–285 (2013). https://doi.org/10.1007/s00604-012-0930-2

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  • DOI: https://doi.org/10.1007/s00604-012-0930-2

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