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Colorimetric determination of 8-hydroxy–2′-deoxyguanosine using label-free aptamer and unmodified gold nanoparticles

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Abstract

We report on a sensitive and specific method for the visual and colorimetric detection of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in human urine by using the 8-OHdG-aptamer (Apt) as the recognition element. The aptamer was adsorbed on the surface of gold nanoparticles which enhances their stability. On addition of 8-OHdG, the conformation of the aptamer changes to form a G-quadruplex structure, thereby losing the ability to protect the nanoparticles and causing a color change from red to blue. The conformational changes were also studied by circular dichroism. The response is linearly dependent on the concentration of 8-OHdG in the range from 5.6 to 282 nM, the limit of detection is 1.7 nM, the relative standard deviation ranges from 1.1 to 4.2 % (for n = 6), and the recoveries range from 95.9 to 104.9 %. The method paves the way for visual analysis of samples without the use of costly instruments.

8-OHdG-aptamer was adsorbed onto the surface of AuNPs which enhances their stability. On addition of 8-OHdG, the conformation of 8-OHdG-aptamer changes to form a G-quadruplex structure, losing the ability to protect the AuNPs and causing a color change of the system.

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Acknowledgments

The authors gratefully acknowledge the support of the National Natural Science Foundation of China (No. 21177052), the Foundation of National 863 Plan of China (No. 2012AA063501), the Science and Technology Program of Hunan Province in China (No. 2010SK3039) and the Construct Program of the Key Discipline (Public Health and Preventive Medicine) in Hunan Province.

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Authors and Affiliations

  1. College of Public Health, University of South China, Hengyang, 421001, People’s Republic of China

    Jia-Cheng Wang, Yong-Sheng Wang, Wei-Qing Rang, Jin-Hua Xue, Bin Zhou, Lu Liu, Qiu-Mei Qian, Yong-Song Wang & Ji-Cheng Yin

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  1. Jia-Cheng Wang
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Correspondence to Yong-Sheng Wang.

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Wang, JC., Wang, YS., Rang, WQ. et al. Colorimetric determination of 8-hydroxy–2′-deoxyguanosine using label-free aptamer and unmodified gold nanoparticles. Microchim Acta 181, 903–910 (2014). https://doi.org/10.1007/s00604-014-1173-1

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  • DOI: https://doi.org/10.1007/s00604-014-1173-1

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